Modification of polycaprolactone scaffolds with vascular endothelial growth factors for potential application in development of tissue engineered vascular grafts

Abstract

In this study, we investigated a biological activity of nonwoven polycaprolactone scaffolds for controlled delivery of vascular endothelial growth factors. The tube scaffolds with incorporated vascular endothelial growth factors were fabricated by method of electrospinning. The polycaprolactone scaffold containing growth factor provided a morphology similar to the native extracellular matrix. The sustained release of biologically active growth factor from scaffold was observed for 80 days The assessment of adhesion and proliferation of multipotent mesenchymal stem cells and endothelial cells on the material surface showed that scaffolds with vascular endothelial growth factors are able to maintain the cellular activity. Results of study demonstrated that incorporated growth factors provide active proliferation of endothelial cells on porous material and cells penetration inside the scaffold. This approach to the creation of a biologically active environment in the scaffold has a great potential in the development of grafts for blood vessels regeneration

Keywords

polycaprolactone / vascular endothelial growth factor / electrospinning / polymeric scaffold

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