Prospects of EPR spectroscopy to the intensity of free radical processes evaluation in tissue-engineering esophagus and diaphragm

Abstract

Tissue engineering can become an alternative option of treatment which is focused on restoration, replacement and regeneration of cells, tissues and failed organs. One of the tasks of regenerative medicine is creation of biological or artificial scaffolds reproducing structure of native tissue and possessing the required physical, chemical and mechanical properties for ensuring cell adhesion and formation of three-dimensional tissue as a result of recellularization with autologous cells. Biological scaffolds must be decellularized to become nonimmunogenic; however this process has to be aimed at the maximum preservation of biochemical composition, morphological structure, and maintenance of the biomechanical properties of the obtained extracellular matrix similar to the properties of native tissue One of the main issues is development of criteria of quality of the obtained scaffolds. In this work the spectroscopy EPR (electron paramagnetic resonance) method is used as one of promising biophysical methods of evaluation of decellularization EPR-spectroscopy applicability is also shown for evaluation of preservation of native organs at a cryopreservation which might already be the first stage of decellularization that is indirectly confirmed by the conducted researches

Keywords

tissue engineering / oesophagus / diaphragm / decellularization / extracellular matrix / biological scaffold / extracellular matrices proteins / EPR spectroscopy

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