Isolation, analysis and application of authogenic adipose derived multipotential mesenchymal stromal cells from dog for therapy pseudoarthrosis of tibial bone

E. Y Zakirova; M. N Zhuravleva; R. F Masgutov; RA. A Usmanov; AA. A Rizvanov

doi:10.23868/gc120310

Genes & Cells ›› 2014, Vol. 9 ›› Issue (3) : 70 -75. DOI: 10.23868/gc120310

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Isolation, analysis and application of authogenic adipose derived multipotential mesenchymal stromal cells from dog for therapy pseudoarthrosis of tibial bone

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Abstract

This study demonstrates the possibility of using cell-based therapies for the treatment of the longterm intergrows fractures and pseudoarthrosis in dogs. Multipotent mesenchymal stem cells (MMSCs) were isolated from adipose tissue of an adult dog. Multipotent cells was confirmed by the ability to differentiate into 3 main directions (osteogenic, adipogenic and chondrogenic), as well as the expression of the markers CD73, CD105, vimentin. Clinical application received MMSCs in the treatment of pseudoarthrosis of tibia in dogs stimulated bone formation processes. The result of cell therapy was callus formation and recovery of the support function of the injured extremity.

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multipotential mesenchymal stromal cells / pseudoarthrosis / dog

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E. Y Zakirova, M. N Zhuravleva, R. F Masgutov, RA. A Usmanov, AA. A Rizvanov. Isolation, analysis and application of authogenic adipose derived multipotential mesenchymal stromal cells from dog for therapy pseudoarthrosis of tibial bone. Genes & Cells, 2014, 9(3): 70-75 DOI:10.23868/gc120310

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