Transformed lung epithelial cells produce microbial RNAse (binase)

Abstract

Today, the targeted drug delivery to tumors is one of the leading researches in anticancer therapy. Using gene engineering techniques the new expression system based on pCS2+ vector was created. Gene of Bacillus pumilis ribonuclease - binase, and gene of intracellular inhibitor of Bacillus amyloliquefaciens - barstar were cloned under promotor of cytomegalovirus. The possibility of binase's gene expression in transformed murine lung epithelial cells was shown. The new plasmid system provides selective cytotoxic action of binase against target tumor cells due to high expression level of foreign protein in transformed cells.

Keywords

pCS2 + / MLE-12 / binase / expression system / pCS2 + / MLE-12 / secretion

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