Evaluation of chondrogenic potential of human dermal fibroblasts after modification with differentiation media and cytokine TGF-β3

Abstract

BACKGROUND: Hyaline cartilage is an avascular tissue that envelopes the surface of major joints. The regenerative capacity of this tissue is restricted due to its high content of extracellular matrix proteins and its modest number of cells. Articular cartilage recovery remains relevant and yet unresolved to date. Utilizing biomedical products that contain modified cells is a promising approach.

AIM: To examine the effects of various culture media on the chondrogenic modification of cells and to compare the results.

MATERIALS AND METHODS: Human dermal fibroblasts and rabbit multipotent mesenchymal stromal cells were modified using the chondrogenic differentiation medium StemPro or recombinant TGF-β3 protein. Alterations in the expression of genes involved in chondrogenesis (Acan, Tgfβ3, Col2α1, Comp) were assessed using the real-time polymerase chain reaction method ΔΔCt.

RESULTS: It was demonstrated that human dermal fibroblasts can induce chondrogenic modification when used in protein media. This cell type is easy to harvest, does not necessitate special cultivation conditions, is readily scalable, and is suitable for allogeneic transplantation.

CONCLUSION: The obtained data can be employed to develop tissue engineering products for the regeneration of hyaline cartilage using allogeneic dermal fibroblasts.

Keywords

tissue engineering / real-time PCR / multipotent mesenchymal stromal cells / MMSCs / TGF-β3

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