EXPRESSION OF OCT-4, P53, P16 AND KI-67, WITH HPV ASSOCIATED PRECANCER AND CANCER OF THE CERVIX

Abstract

The pathology of cervical HPV infection is detected by three types of epithelial-mesenchymal spheroidal structures: reparative, neoplastic and cancerous involved in reparation processes and in tumor growth. Cancer spheroids are different from neoplastic not only larger in size but also a tendency to merge, and high expression of p53. Expression of tumor markers p53, p16 and Ki-67 could be used in the early differential diagnosis of precancer and squamous cell carcinoma of the cervix.

Keywords

p53 / p16 / Ki-67 / cervical cancer / p53 / p16 / Ki-67 / HPV

References

[1] Новик В.И. Скрининг рака шейки матки. Практическая онкология, 2010, 11(2): 66-73.

[2] Jacobs MV, Snijders PJ, Voorhorst F J, et al. Reliable high risk HPV DNA testing by polymerase chain reaction: an intermethod and intramethod comparison. Journal of clinical pathology, 1999, 52(7): 498-503.

[3] Beachy PA, Karhadkar SS, Berman DM. Tissue repair and stem cell renewal in carcinogenesis. Nature, 2004, 432(7015): 324-31.

[4] Reya T, Morrison S J, Clarke MF, et al. Stem cells, cancer, and cancer stem cells. Nature, 2001, 414(6859): 105-111.

[5] Schlessinger D, Van Zant G. Does functional depletion of stem cells drive aging?. Mechanisms of ageing and development, 2001, 122(14): 1537-53.

[6] Crum CP. Contemporary theories of cervical carcinogenesis: the virus, the host, and the stem cell. Modern Pathology, 2000, 13(3): 243-51.

[7] Regezi J A, Zarbo R J, Regev E, et al. p53 protein expression in sequential biopsies of oral dysplasias and in situ carcinomas. Journal of oral pathology & medicine, 1995, 24(1): 18-22.

[8] Wang N, Yao Q, Li X, et al. Detection of HPV and p53 gene expressions in cervical cancer tissue[J]. Medical Journal of Qilu, 2004, 20(2): 98-100,102.

[9] Scheffner M, Huibregtse JM, Vierstra RD, et al. The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Cell, 1993, 75(3): 495-505.

[10] Liu Y, He Y, Jin A, et al. Ribosomal protein- Mdm2-p53 pathway coordinates nutrient stress with lipid metabolism by regulating MCD and promoting fatty acid oxidation. Proceedings of the National Academy of Sciences, 2014, 111(23): E2414-E2422.

[11] Vousden KH, Ryan KM. p53 and metabolism. Nature Reviews Cancer, 2009, 9(10): 691-700.

[12] E.A. Kogan, PhD, ScD; N.M. Fayzullina, PhD; T.A. Demura, PhD; G.T. Sukhikh, PhD, ScD. Reparative Spheroids in HPV-Associated Chronic Cervicitis. Science Center of obstetrics, gynecology and perinatology n.a. acad. V.I. Kulakov Moscow, RF. International Journal of BioMedicine 2013; 3(3): 192-6.

[13] Castle P.E., Sadorra M., Lau T., et al. Evaluation of a prototype real-time PCR assay for carcinogenic human papilloma virus (HPV) detection and simultaneous HPV genotype 16 (HPV16) and HPV18 genotyping // J Clin Microbiol 2009; 47: 3344-47.

[14] Коган Е.А., Файзуллина Н.М., Ли Ц. и др. Эффективность диагностики цервикальной интраэпителиальной неоплазии шейки матки с использованием комплекса методов: жидкостной цитологии, двойного иммуноокрашивания p16/Ki67 и ВПЧ тестирования // Акушерство и гинекология. 2014. №7. С. 43-47.

[15] Storey A, Thomas M, Kalita A, et al. Role of a p53 polymorphism in the development of human papilloma-virus-associated cancer. Nature 1998; 393(6682): 229-34.

RIGHTS & PERMISSIONS

Kogan E.A., Ts L.T., Faizullina N.M., Kazachenko A.V.