Diagnosis and treatment of fungal keratitis. Part I

Abstract

Fungal keratitis (FK) is a difficult diagnostic challenge for ophthalmologists.

The aim is to familiarize practicing physicians with the diagnostic algorithm worked out in the Ophthalmological Center of SPB City hospital No. 2 using modern research methods, and to assess the epidemiology of fungal keratitis in the North-West Region.

Materials and methods. Patients underwent laboratory diagnostics (fluorescence microscopy of corneal scrapings from the cornea, сulture on Sabouraud agar and broth), confocal in vivo microscopy, optical coherence tomography.

Results. During the period from 2007 to 2017, 41 cases of FK were identified in the City hospital No. 2, of which filamentous fungi were the causative agent in 32 cases (78%), yeast fungi — in 9 cases (22%). Our analysis included patients with fungal keratitis over the past three years, all of them underwent a full diagnostic cycle. Filamentous fungi were found among 12 of them (63%), yeast — in 7 (37%). Our data, considering the statistics of fungal keratitis in the North-West of Russia — a region with a high level of urbanization and industrialization, and located in the temperate zone — showed a predominance of filamentous fungi as pathogens (prevalence 1.3 times higher). Our scheme of keratitis diagnostics — confocal in vivo microscopy, OCT, fungal culture — is a reliable way to identify fungal pathogens in the cornea, and can be recommended for use in practical ophthalmology.

Keywords

fungal keratitis / epidemiology / laboratory diagnostics / confocal microscopy / optical coherence tomography

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RIGHTS & PERMISSIONS

Skryabina Y.V., Astakhov Y.S., Konenkova Y.S., Kasymov F.O., Zumbulidze N.G., Varganova T.S., Petukhov V.P., Pirgunova A.A., Masian J., Klimko N.N., Bogomolova T.S., Desyatik E.A.