The use of platelet lysate to increase the growth-stimulating effect of the amniotic membrane in vitro

Abstract

AIM: To work out the technique of saturation of the preserved amniotic membrane (AM) with platelet rich plasma (PRP) lysate and to evaluate the growth-stimulating effect of a combination of AM and PRP lysate in vitro.

MATERIALS AND METHODS: In the experiment, AM samples preserved in 3 ways were used: silicate drying, lyophilization, cryopreservation. PRP lysate was prepared on the basis of volunteers’ blood. During the exposure of AM with PRP lysate, the optimal saturation time of canned AM with lysate was determined, the volume of lysate that 1 cm² of AM could adsorb was estimated. The growth-stimulating effect of AM transplants was evaluated in the culture of human buccal epithelium. The dynamics of cell growth was evaluated after 1, 2 and 3 days from the moment of sowing.

RESULTS: In the presence of PRP lysate, the mass of silicate–dried AM increased 4.2 times, lyophilized AM — 4.8 times, cryopreserved AM — 1.8 times. AM samples obtained by lyophilization adsorbed PRP lysate most effectively. Five minutes of exposure with PRP lysate are enough to fully saturate the AM. AM without PRP lysate did not give a growth-stimulating effect.

CONCLUSIONS: When comparing experiments with PRP lysate without AM and AM with PRP lysate, it was found that the greatest stimulation of cell growth occurred when using lyophilized AM and PRP lysate. Saturation of cryopreserved AM with PRP lysate was ineffective, and when using silicate-dried AM impregnated with PRP lysate, the greatest growth-stimulating effect was observed on the 1^st day.

Keywords

platelet-rich plasma / lysate / amniotic membrane / growth-stimulating effect / growth factors

References

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RIGHTS & PERMISSIONS

Bozhenko D.A., Chentsova E.V., Borovkova N.V., Ponomarev I.N., Storozheva M.V., Makarov M.S., Makarov P.V.