The content of matrix metalloproteinase-9 in aqueous humor of the eyes in children with endogenous uveitis

Abstract

AIM: This study aimed to determine the content of matrix metalloproteinase-9 (MMP-9) in the aqueous humor (AH) of the eyes in children with uveitis and its role during the disease.

MATERIAL AND METHODS: Twenty children (20 eyes with uveitis) aged from 3 to 16 yr (11.25±3.43 yr on average) and three children with congenital cataract from the control group were examined. The concentration of MMP-9 was determined by enzyme-linked immunosorbent assay (ELISA) using the ELISA kit for MMP-9 (Cloud-Clone Corp, USA). The optical density of the samples was measured using a multifunctional photometer for Synergy microplates (BioTek, USA).

RESULTS: Children with uveitis had a higher content of MMP-9 in the AH than children in the control group (p=0.006). An increase in the content of MMP-9 in the AH of the eyes was correlated with an increase in the degree of proliferative changes in the eye.

CONCLUSION: The concentration of MMP-9 in the AH of the eyes correlates with the severity of proliferation, and its measurement can be used to assess the severity of the inflammatory process in the eye.

Keywords

uveitis / matrix metalloproteinase-9 / children / aqueous humor

References

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RIGHTS & PERMISSIONS

Katargina L.A., Chesnokova N.B., Denisova E.V., Khrabrova M.A., Beznos O.V.