Ambiguity effects of recombinant interferon α2B in an in vitro experiment on the expression of nuclear factor NF-KB, IFNα’βR AND IFNγR (CD119) receptors of neutrophilic granulocytes of patients with chronic herpes virus infections

Abstract

Introduction. Neutrophil granulocytes (NGs) and the interferon’s system (IFN) play a leading role in antiviral immune defense. At the same time, patients suffering from atypical chronic active herpes-viral infections (ACA-HVI) often have neutropenia and impaired induced IFNα/β and IFNγ production. It is possible that these patients have various disorders of intracellular signaling at all stages of IFNα/β synthesis by immune system cells, including NG, which leads to IFNα/β deficiency. An adequate level of nuclear factor NF-kB expression has positive effects on synthesis of IFNα/β in the last stages of signaling. The disturbances in the expression of NF-kB can lead to defects in the synthesis of IFNα/β.

The aim: to clarify the features of nuclear factor NF-kB, membrane receptors IFNα|βR and IFNγR (CD119) expression on NG of patients with AСA-HVI, with the subsequent assessment of the recombinant IFNα2b (recIFNα2b) effects on them in the experimental system in vitro.

Materials and methods: 25 patients with ACA-HVI of both sexes aged 23-64 years were included in the main group of the study (MSG). In the complex of the study for the detection of herpes-viral infections: the method of serodiagnosis (ELISA), for the detection of the genome of viruses - PCR-RT. 407 blood samples were examined in the in vitro system. Flow cytofluorimetry was used to estimate the amount (%) of NG expressing NF-kB, IFNα|βR, IFNγR, and their expression levels before and after incubation with recIFNα2b. Adequate statistical methods were applied.

Results: In patients suffering from ACA-HVI, a deficiency of induced IFNα and IFNγ production was detected, due to a decrease in the expression density of nuclear factor NF-kB NG, as well as a violation of the expression of membrane receptors IFNα|βR, IFNγR. RecIFNα2b in the in vitro system had a mixed effect on the expression of NF-kB, IFNα|βR and IFNγR NG.

Conclusion: The deficiency of induced IFNα and IFNγ production in patients with ACA-HVI is associated with variable changes in NF-kB, IFNα|βR and IFNγR NG expression. RecIFNα2b in the in vitro system has an ambiguous effect on altered expression of NF-kB, IFNα/β and IFNγ NG, which appears to depend on the innate or adaptive nature of these disorders.

Keywords

herpesvirus infections / interferon system / nuclear factor NF-kB / neutrophilic granulocytes

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RIGHTS & PERMISSIONS

Nesterova I.V., Khalturina E.O., Nelyubin V.N., Khaidukov S.V., Chudilova G.A., Malinovskaya V.V.