Comparison of laboratory diagnostic methods for urogenital infections caused by Chlamydia trachomatis

Abstract

According to the Russian-Swedish project there was performed a comparison of methods used for Chlamydia trachomatis detection in cervical samples, obtained from 397women and urethral samples from 253 men. All specimens were examined by direct immunofluorescence (DIF), polymerase chain reaction (PCR) and cell culture (CC). In high-prevalence group (group I) chlamydiae were detected in 17,8% and 28,0% of cases in men and women, respectively. Ingroup II containing patients who were subjected to screening examination, chlamydiae were found in 5% of cases both in men and women. PCR was shown to be the most sensitive when cervical samples in group I and cervical and urethral samples in group II were investigated. When urethral samples in group I were tested, DIF proved to have the highest sensitivity. All the methods used were found to be high specific. The search for standards of genital chlamydial infection diagnosis is in progress.

Keywords

Chlamydia trachomatis / urogenital infections / direct immunofluorescence method

References

[1] Emma R., Lliffe-Lee, Grant McClarty. Glucose metabolism in Chlamydia trachomatis: the «energy parasite» hypothesis revisited // J. Mol. Microbiol. — 1999. — Vol. 33, № 1. - P. 177-187.

[2] Moulder J. И/. Interaction of chlamydiae and host cells in vitro // Rev. Microbiol. — 1991. - Vol. 55. — P. 143-190.

[3] Domeika M., Mardh P.-А. ABC on Chlamydia. Continuing education for laboratory professionals. — Sweden, Uppsala, 1993.

[4] Global prevalence and incidence of selected STIs. Overview and estimates. — WHO, Geneva, 2001.

[5] Greedall G. A., Haas S. T., Holbrook K., Walsh B., et al. The relationship of Chlamydia trachomatis infection and male infertility // Am. J. Public Health. — 1993. - Vol. 83, № 7. - P. 996- 1001.

[6] Fish A. N. J., Fairweather D. У. I., CrielJ. D., Ridgway G. L. Chlamydia trachomatis infection in gynaecology clinic population: Identification of high-risk groups and the value of contact tracing // Eur. J. Obstet. Gynecol. — 1989. — Vol. 31, № 1. — P. 67-74.

[7] Jbarra C. A., Lopez 0. G., Fueste G. L. D. Un ensayo para el aislamlento у demonstracion de Chlamydia trachomatis en exodados uretrales // Rev. Latinoamer.Microbiol. — 1986. — Vol. 26, №2. — P. 95-98.

[8] Henry-SuchetJ., Catalan F., Loffredo V., et al. Chlamydia trachomatis associated with chronic inflammation in abdominal specimens from women selected for tuboplasty // Fertil. Steril. — 1981. — Vol. 36, № 5/6. - P. 599-605.

[9] Савичева A. M. Акушерские и микробиологические аспекты патогенеза и диагностики генитального хламидиоза: Автореф. дис. ... д-ра мед. наук. — СПб, 1991.

[10] Domeika М. A. Diagnosis of infections due to Chlamydia trachomatis // Scand. J. Infect. Dis. - 1997.- Vol. 76 (Suppl.). - P. 121-127.

[11] Chlamydia trachomatis infections in Eastern Europe / Eds. M. Domeika, A. Hallen. — KATA Studio. Kaunas, 2000.

[12] Domeika M. A., Ganusauskas A., BassiriM., FrpmanG., Merdh P.-A. Comparison of polymerase chain reaction, direct immunofluorescence, cell culture and enzyme immunoassay for the detection of Chlamydia psittaci in bull semen // J. Vet. Microbiol. — 1994. - Vol. 42. - P. 273-280.

[13] Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noorden J. Rapid and simple method for purification of nucleic acids // J. Clin. Microbiol. - 1990. - Vol. 28, № 3. - P. 495-503.

[14] Ossewaade J. M., et al. Development and clinical evaluation of a polymerase chain reaction test for detection of Chlamydia trachomatis // J. Clin. Microbiol. — 1992. - Vol. 30. - P. 2122-2128.

[15] Ripa K. T, MardhP.-A. Cultivation of Chlamydia trachomatis in cycloheximide treated McCoy cells //J. Clin. Microbiol. - 1977. - Vol. 44. - P. 1-5.

[16] Domeika M. Diagnosis of genital chlamydial infections in humans as well as in cattle. — Sweden, Uppsala, 1994. — P. 47.

[17] Savicheva A., ChalepoK., Chypitsina E., HerrmanB., HjelmE., Hjalmarsson S., Domeika M. Evaluation of methods used for diagnosis of Chlamydia trachomatis infections in St.-Petersburg: the pilot study//Int. J. STD AIDS (Int. Congress of STI, 24-27 June, 2001, Berlin). — Vol. 12 (Suppl. 2). — P. 114.

[18] Domeika M., DrulyteO., SenkieneL., Spukaite T. Genital Chlamydia trachomatis infections in Lithuania. Proceedings of the Third Meeting of the European Society for Chlamydia Research. — Austria, Vienna, 1996, September 11-14. - P. 396.

[19] Domeika M., ButylkinaR., НаНйп A., Spukaite T, et al. Prevalence of Chlamydia trachomatis infections in women attending six women’s health care units in Kaunas, Lithuania // Sex. Transm. Dis. — 2001.

[20] JaschekG., Gaydos L. E., Welsh L. E., Quitt T. Direct detection of C. trahomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay// J. Clin. Microbiol. — 1993. — Vol. 31.-P. 1209-2112.

[21] Caul E. O., Herring A. J. The diagnosis of Chlamydia trachomatis genital infection // International handbook of chlamydia / T. R. Moss (ed.). — 2001. — Euro med Communications Ltd, Exeter. — P. 21-33

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