Evaluation of a real-time nucleic acid sequencebased amplification (nasba) assay for diagnosis of urogenital chlamydial infection

Е. V. Shipitsyna; А. М. Savicheva; N. E. Vorobyova; Е. V. Sokolovsky; А. Е. Guschin; Р. G. Ryzhikh; G. A. Shipulin; P. N. Krotin; L. V. Merculova; О. Y. Landina

doi:10.17816/JOWD83578

Journal of obstetrics and women's diseases ›› 2005, Vol. 54 ›› Issue (4) : 17 -21. DOI: 10.17816/JOWD83578

Original study articles

research-article

Evaluation of a real-time nucleic acid sequencebased amplification (nasba) assay for diagnosis of urogenital chlamydial infection

Author information +

History +

PDF

Abstract

This study was aimed to evaluate a new realtime nucleic acid sequence-based amplification (NASBA-Real- Time) assay for diagnosis of urogenital Chlamydia trachomatis infection. A total of 193 patients aged 16 to 42 (mean age, 22,8 years) were examined, with most of them having symptoms of urogenital infection. Cervical and urethral swabs from women and men, respectively, were investigated with the use of cell culture method, polymerase chain reaction (PCR) and NASBA- Real-Time. C. trachomatis infection was diagnosed in 29 patients (15 %): in 21 patients - by all the three methods, whereas 8 samples were culture negative. Sensitivity of PCR and NASBA- Real-Time methods were 100 %, cell culture method - 78,4 %. Negative predictive value of PCR and NASBA-Real-Time was found to be 100 %, and that of cell culture - 95,3 %. Specificity as well as positive predictive values of all the three methods equaled 100%. Thus, the new real-time NASBA-Real-Time assay was shown to be a very sensitive and specific test, which can be recommended as a confirmatory method for diagnosis of urogenital chlamydial infection.

Keywords

Chlamydia trachomatis / 16S rRNA / NAAT / PCR / NASBA-Real-Time

Cite this article

Download citation ▾

Е. V. Shipitsyna, А. М. Savicheva, N. E. Vorobyova, Е. V. Sokolovsky, А. Е. Guschin, Р. G. Ryzhikh, G. A. Shipulin, P. N. Krotin, L. V. Merculova, О. Y. Landina. Evaluation of a real-time nucleic acid sequencebased amplification (nasba) assay for diagnosis of urogenital chlamydial infection. Journal of obstetrics and women's diseases, 2005, 54(4): 17-21 DOI:10.17816/JOWD83578

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	An Q., Radcliffe G., Vassallo R., et al. Infection with a plasmid- free variant of chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection//J. Clin. Microbiol. - 1992. - Vol. 30. - P. 2814-2821.

[2]	Battle T.J., Golden M.R., Suchland K.L., et al. Evaluation of laboratory testing methods for Chlamydia trachomatis infection in the era of nucleic acid amplification//J. Clin. Microbiol. - 2001. - Vol. 39. - P. 2924-2927.

[3]	Black C.M., Marrazzo J., Jonson R.E., et al. Head-to-head multicenter comparison of DNA probe and nucleic acid amplification tests for Chlamydia trachomatis infection in women performed with an improved reference standard//J. Clin. Microbiol. — 2002. - Vol. 40. - P. 3757-3763.

[4]	Deiman B., van Aarle P., Sillekens P. Characteristics and applications of nucleic acid sequence-based amplification (NASBA)//Mol. Biotech. - 2002. - Vol. 20. - P. 163-179.

[5]	Johnson R.E., Newhall W.J., Papp J.R., et al. Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections//Morbid. Mortal. Weekly Reports. - 2002. - Vol. 51 (RR-15). - P. 1-38.

[6]	Leone G., van Schijndel H., van Gemen B., et al. Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA//Nucleic Acids Res. - 1998. - Vol. 26. - P. 2150-2156.

[7]	Mahony J.B., Song X., Chong S., et al. Evaluation of the NucliSens Basic kit for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in genital tract specimens using nucleic acid sequence-based amplification of 16S rRNA//J. Clin. Microbiol. - 2001. - Vol. 39. - P. 1429- 1435.

[8]	McAdam A.J. Discrepant analysis: how can we test a test?//J. Clin. Microbiol. - 2000. - Vol. 43. - P. 2027-2029.

[9]	Morre S., Sillekens P., Jacobs M.V., et al. RNA amplification by nucleic acid sequence-based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples//J. Clin. Microbiol. - 1996. - Vol. 34. - P. 3108-3114.

[10]	Newhall W.J., Johnson R.E., Delisle S. et al. Head-to-head evaluation of five Chlamydia tests relative to a quality-assured culture standard//J. Clin. Microbiol. - 1999. - Vol. 37. - P. 681-685.

[11]	Stary A. Diagnosis of genital chlamydial infections in the era of amplification technologies//In: Proceedings of the Fifth Meeting of the Society for Chlamydia Research (Deak J. ed.). - Budapest: University of Szeged. - 2004. - P. 61-63.

[12]	Weusten J.J., Carpay W.M., Oosterlaken T. et al. Principles of quantification of viral load using Nucleic Acid Sequence-Based Amplification in combination with homogeneous detection using molecular beacons//Nucleic Acids Res. - 2002. - Vol. 30.-P. 6-26.