The analysis of group в streptococcal clinical strains for the presence of the genes encoding for potential adherence factors and localized on the «pathogenicity islands»

A. N. Suvorov , А. М. Savicheva , А. V. Glushanova , К. А. Oganyan , К. В. Grabovskaya , О. V. Alaiceva , S. L. Zatsiorskaya , D. J. , О. N. Arjanova

Journal of obstetrics and women's diseases ›› 2005, Vol. 54 ›› Issue (2) : 50 -55.

PDF
Journal of obstetrics and women's diseases ›› 2005, Vol. 54 ›› Issue (2) : 50 -55. DOI: 10.17816/JOWD81945
Original study articles
research-article

The analysis of group в streptococcal clinical strains for the presence of the genes encoding for potential adherence factors and localized on the «pathogenicity islands»

Author information +
History +
PDF

Abstract

The collection of group В streptococcal clinical strains has been analyzed for the presence of genes encoding for putative adherence and aggregation factors. The presence of the genes designated as sspB1 and sspB2 were tested by PCR and DNA hybridization. The genes under study located in GBS genome on “pathogenicity islands” were found to be heterogeneous and could be associated with urological or invasive diseases. No significant correlation between the GBS serotype and sspB genes pattern have been discovered.

Keywords

group В streptococci / sspB1 and sspB2 genes / PCR / DNA hybridization

Cite this article

Download citation ▾
A. N. Suvorov, А. М. Savicheva, А. V. Glushanova, К. А. Oganyan, К. В. Grabovskaya, О. V. Alaiceva, S. L. Zatsiorskaya, D. J., О. N. Arjanova. The analysis of group в streptococcal clinical strains for the presence of the genes encoding for potential adherence factors and localized on the «pathogenicity islands». Journal of obstetrics and women's diseases, 2005, 54(2): 50-55 DOI:10.17816/JOWD81945

登录浏览全文

4963

注册一个新账户 忘记密码

References

[1]

Adderson Е.Е., Takahash S., WangY. et al. Subtractive hybridization identifies a novel predicted protein mediating epithelial cell invasion by virulent serotype III group В Streptococcus agalactiae // Infect. Immun.—2003.—Vol. 71.—P. 6857—6863.

[2]

Bake C.J. and Edwards M.S. Group В streptococcal infection. In Infectious diseases of the fetus and the newborn infant // Remington J.S. and Klein J.O. eds. (Philadelphia: W.B. Saunders Company).—1995. —P. 980—1054.

[3]

Chmourygina I., Suvorov A.N., Ferrieri P. et al. //Infect. Immun.—1996.—Vol. 64.—P. 2387—2390.

[4]

DemuthD.R., Duan Y., Brooks W. etal. Tandem genes encode cell—surface polypeptides SspA and SspB which mediate adhesion of the oral bacterium Streptococcus gordonii to human and bacterial receptors // Mol. Microbiol.—1996.—Vol. 20.—P. 403—413.

[5]

Embleton N., Wariyar U., Hey E. et al. Mortality from early onset group В streptococcal infection in the United Kingdom // Arch. Dis. Child. Fetal Neonatal. Ed.—1999.—Vol. 80.—P.139—141.

[6]

Gardam M.A., Low D.E., Saginur R.et al. Group В streptococcal necrotizing fasciitis and streptococcal toxic shock—like syndrome in adults // Arch. Intern. Med. — 1998.—N 24.—Vol. 158.—P. 1704—1708.

[7]

Gibson R.L., Lee M.K., Soderland C.et al. Group В streptococci invade endotelial cells: type III capsular polysacharide attenuates invasion // Infect. Immun.—1993.—Vol. 61.—P. 478—485.

[8]

Glaser, Rusniok C., Buchrieser C. et al. Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease // Molec. Microbiol.—2002.—Vol. 45.—P. 1499—1514.

[9]

Henderson I.R., Navarro—Garcia F., Desvaux M. et al. // Microbiol. Mol. Biol. Rev.—2004.—Vol. 68.—P. 692—744.

[10]

Kong F., Gowan S., Martin D. et al. Molecular profiles of group В streptococcal surface protein antigen genes: relationship to molecular serotypes //J. Clin. Microbiol.—2002.—Vol. 40.—P. 620—626.

[11]

Lukacs S.L., Schoendorf K.C., Schuchat A. et al. Trends in sepsis—related neonatal mortality in the United States, 1985—1998 // Pediatr. Infect. Dis. J.—2004.—Vol. 23.—P. 599—603.

[12]

Maniatis T., Fritsch E.F., Sambrook J. Molecular Cloning: A Laboratory Manual. — Cold Spring Harbor Laboratory Press, Cold Spring Harbor: NY, 1982.—P. 1—545.

[13]

Suvorov A.N., Sverdlova A., Totolian A.A. et al. //Med. Microbiol. Lett.—1996.—Vol. 4.—P. 341—345.

[14]

Suvorov A.N., Ferretti J.J. Construction of a GBS—GAS DNA subtraction library allows discovery of previously unidentified GBS genes and rapid location of unique regions on the GBS chromosome // J. Basic Microbiol.—2004. — Vol. 44.—P. 66—74.

[15]

Tettelin H., Masignani V., Cieslewicz M.J. et al. //Proc. Natl. Acad. Sci. USA.—2002.—Vol. 99.—P. 12391—12396.

RIGHTS & PERMISSIONS

Eсо-Vector

AI Summary AI Mindmap
PDF

50

Accesses

0

Citation

Detail

Sections
Recommended

AI思维导图

/