Free radical reactions in the development of combined hyperplastic processes in female reproductive system organs

Abstract

Intact myometrium specimen, received from 56 women of reproductive age during surgical interventions. There were 30 women with combined hyperplastic processes in the reproductive system organs (uterine myoma, endometrium hyperplasia, adenomyosis, benign ovarian tumors, mastopathy, background cervical pathology), and in 26 cases solitary uterine myoma was found. At infrared spectrometry of the lyofilized myometrium, taken from patients with combined proliferation foci, a stable peak with wave number 3500 sm^—1 was found, which reflects the accumulation of molecules with oxidized OH—groups and a change in proportion of OH—/NH—containing molecules. At the study of the myometrium of patients with single myoma focus the described peak was absent. The acquired data suggests, that combined benign hyperplasias in the reproductive system organs are accompanied by the oxidative stress cascade reactions, characterized by uncontrollable production of free radicals and/or insufficiency of antiradical protection mechanisms.

Keywords

oxidative stress / combined hyperplastic processes

References

[1] Ванин А.Ф. Оксид азота: регуляция клеточного метаболизма без участия системы клеточных рецепторов // Биофизика.—2001.—Т. 46, Вып. 4.—С. 631—641.

[2] Меньшикова Е.Б., Зенков Н.К., Реутов В.П. Оксид азота и NO—синтазы в организме млекопитающих при различных функциональных состояниях // Биохимия. — 2000.— Т. 65, Вып. 4. — С. 485_503.

[3] Плужников Н.Н., Чиж С.И., Юзвинкевич Л. С. и др. Оксидативный стресс. Фундаментальные и прикладные проблемы // Актуальные проблемы и перспективы развития военной медицины: науч. тр. / НИИЦ (МБЗ) ГНИИИВМ МО РФ.—СПб., 2000.—Т. 2.—С. 193—223.

[4] Плужников Н.Н., Гайдар Б.В., Чепур С.В. Редоксрегуляция: фундаментальные и прикладные проблемы // Актуальные проблемы и перспективы развития военной медицины: научн. тр. / НИИЦ (МБЗ) ГНИИИВМ МО РФ.—СПб., 2003.—Т. 4.—С. 139—173.

[5] Adler V., Yin Z., Funch S. Y. et al. Regulation of JNK signaling by GSTp // EMBO J.—1999.—Vol. 18, N 5.—P. 1321—1334.

[6] Beckman J.S., Beckman K.M., Chen J., Marshall P.A. // Proc. Natl. Acad. Sci. USA.—1990.—Vol. 87.—P. 1620—1624.

[7] Dalton T.P., Shertzer H.G., Puga A. // Annu. Rev. Pharmacol. Toxicol.—1999.—Vol. 39.—P. 67—101.

[8] Dery O., Corvesa C.U., Steinoff M., Bunnett N.W. Proteinase—activated receptors: novel mechanism of signaling by serine proteases // Am. J. Physiol.—1998.—Vol. 274, N 6.—P. 1429—1452.

[9] Flohe L., Brigelius—Flohe R., Saliou C. et al. Redox regulation of NF—кВ activation // Free Radic Biol. Med. — 1997.—Vol. 22.—P. 1115—1126.

[10] Guls D., Botero A., Shah S., Curry H.A. //Toxicol. Lett.— 1999.—Vol. 106.—P. 93—106.

[11] Kamata H., Hirata H. Redox regulation of cellular signaling // Cell. Signal.—1999.—Vol. 11.—P. 1—14.

[12] Lander H.M. An essential role for free radicals and derived species in signal transduction // FASEB J.—1997. — Vol. 11. — P. 118—124.

[13] Lee F.S., Hagler J., Chen Z.J., Maniatis T. Activation of the I—кВ kinase complex by MEKK1, a kinase of the JNK pathway // Cell.—1997.—Vol. 88.—P. 213—222.

[14] Muller J., Rupee R.A., Baeuerle P.A. Study of the gene regulation by NF—кВ and AP—1 in responce to reactive oxygen intermediates // Methods. — 1997. — Vol. 11. — P. 301—312.

[15] Xanthoudakis S., Curran T. Redox regulation of AP—1: a link between transcription factor signaling and DNA repair // Adv. Exp. Med. Biol. — 1996. — Vol. 387. —P. 69—75.

RIGHTS & PERMISSIONS

Eсо-Vector