Gas chromatography-mass spectrometry based metabolomics of urine steroids in women with different phenotypes of polycystic ovary syndrome

Abstract

This article presents an analysis of the metabolism of androgens, glucocorticoid hormones, and progestins obtained  by gas chromatography-mass spectrometry in 56 women with obesity and hyperandrogenia in comparison with 23 women with alimentary-constitutional obesity and 25 healthy women. An increase in urinary excretion of androsterone, 16-oxo-androstenediol (16-oxo-dA2), and α-tetrahydrometabolites of cortisone and corticosterone has been established in 25 women with polycystic-altered ovaries according to ultrasound, with urinary excretion of dehydroepiandrosterone (DHEA) and its metabolites (dA2-17β, 16β-DHEA, 16-oxo-dA2, and androstentriol), α- and β-metabolites of glucocorticoids and androstenedione, and 5-en-pregnans increased in 31 women without signs of polycystic ovaries according to ultrasound. A decrease in the tetrahydrocortisol/tetrahydrocortisone ratio was found in women with polycystic ovaries, indicating a decrease in the activity of 11β-hydroxysteroid dehydrogenase type 1. Evidence for the increased 5α-reductase activity was obtained in women with different phenotypes of polycystic ovary  syndrome.

Keywords

hyperandrogenism / obesity / polycystic ovary syndrome / urine steroids metabolism / gas chromatography-mass spectrometry

References

[1] Bala M, Meenakshi KM, Gupta A. Correlation of HbA1C Levels With Body Mass Index in Newly Diagnosed Polycystic Ovary Syndrome. EJIFCC. 2017;28(3):196-204.

[2] Moran C, Arriaga M, Rodriguez G. Obesity Differentially AffectsPhenotypes of Polycystic Ovary Syndrome. Int J Endocrinol. 2012;2012:1-16. doi: 10.1155/2012/317241.

[3] Baldani DP, Skrgatić L, Simunić V, et al. Characteristics of different phenotypes of polycystic ovary syndrome based on the Rotterdam criteria in the Croatian population. Coll Antropol. 2013;37(2):477-82.

[4] Azziz R, Woods KS, Reyna R, et al. The prevalence and features of the polycystic ovary syndrome in an unselected population. J Clin Endocrinol Metabol. 2004;89(6):27-45. doi: 10.1210/jc.2003-032046

[5] Barker DJ, Eriksson JG, Forsén T, Osmond C. Fetal origins of adult disease: strength of effects and biological basis. Int J Epidemiol. 2002;31(6):12-25.

[6] Concha CF, Sir PT, Recabarren SE, Pérez BF. Epigenetics of polycystic ovary syndrome. Rev Méd Chile. 2017;145(7):907-915. doi: 10.4067/s0034-98872017000700907.

[7] Гафарова Е.А. Клинико-лабораторные проявления СПКЯ в зависимости от фенотипических особенностей и уровня витамина D в крови // Практическая медицина. - 2016. - Т. 1. - № 93. - С. 80-84. [Gafarova E.A. Clinical and laboratory manifestations of PCOS depending on phenotypic characteristics and vitamin D blood level. Prakticheskaja medicina. 2016;1(93):80-4. (In Russ.)]

[8] The Rotterdam ESHRE/ASRM-sponsored PCOS consensus workshop group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19(1):41-7.

[9] Murri M, Insenser M, Fernández-Durán E, et al. Effects of polycystic ovary syndrome (PCOS), sex hormones, and obesity on circulating miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 expression. J Clin Endocrinol Metab. 2013;98(11):E1835-44. doi: 10.1210/jc.2013-2218.

[10] Smitka K, Maresova D. Adipose tissue as an endocrine organ an update on pro-inflammatory and anti-inflammatory microenvironment. Prague Medical Report. 2015;116(2):87-111. doi: 10.14712/23362936.2015.49.

[11] Jungheim ES, Travieso JL, Carson KR, Moley KH. Obesity and Reproductive Function. Obstet Gynecol Clin North Am. 2012;39(4):479-93. doi: 10.1016/j.ogc.2012.09.002.

[12] Jung CH, Kim MS. Molecular mechanisms of central leptin resistance in obesity. Arch Pharm Res. 2013;36(2):21-7. doi: 10.1007/s12272-013-0020-y.

[13] Welsh S, Whigham LD, Maxwell R, Lindheim SR. A review of the impact of obesity on reproduction and potential barriers in conveying the message. Gynecol Obstet Res Open J. 2016;SE(3):S1-S7. doi: 10.17140/GOROJ-SE-3-101.

[14] Chennaf C, Yahia M, Bouafia W, et al. Impact of Obesity on Fertility in a Population of Women in the Wilaya of Batna. International Journal of Medical, Health, Pharmaceutical and Biomedical Engineering. 2014;8(2):105-8.

[15] Zera C, McGirr S, Oken E. Screening for obesity in reproductive-aged women. Prev Chronic Dis. 2011;8(6):1-5.

[16] Jungheim ES, Macones GA, Odem RR, et al. Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during in vitro fertilization. Fertil Steril. 2011;95(6):17-35. doi: 10.1016/j.fertnstert.2011.01.154.

[17] Jungheim ES, Macones GA, Odem RR, et al. Elevated serum alpha-linolenic acid levels are associated with decreased chance of pregnancy after in vitro fertilization. Fertil Steril. 2011;96(4):880-3. doi: 10.1016/j.fertnstert.2011.07.1115.

[18] Echiburu B, Perez-Bravo F, Galgani JE, et al. Enlarged adipocytes in subcutaneous adipose tissue associated to hyperandrogenism and visceral adipose tissue volume in women with polycystic ovary syndrome. Steroids. 2018;130:15-21. doi: 10.1016/j.steroids.2017.12.009

[19] Sang Q, Li X, Wang H, et al. Quantitative methylation level of the EPHX1 promoter in peripheral blood DNA is associated with polycytic ovary syndrome. PLoS One. 2014;9(2):e88013. doi: 10.1371/journal.pone.0088013.

RIGHTS & PERMISSIONS

Matyushenko M.V., Vorokhobina N.V., Velikanova L.I., Kovalyova Y.V.