Comparison of laboratory methods for diagnosis of Trichomonas vaginalis
Zinaida Mikhaylovna Martikaynen , Aleksey Nikolayevich Grigoryev , Olga Sergeyevna Ryzhkova , Yelena Vasilyevna Shipitsyna , Alevtina Mikhaylovna Savicheva , Irina Viktorovna Litvinenko , Alla Valentinovna Galkina , Nina Igorevna Kudryavtseva , Yelena Mikhaylovna Tangatarova , Olga Aleksandrovna Lomakina , Yelena Petrovna Protasova , Svetlana Yuryevna Romantseva
Journal of obstetrics and women's diseases ›› 2014, Vol. 63 ›› Issue (1) : 5 -9.
Comparison of laboratory methods for diagnosis of Trichomonas vaginalis
The paper presents data on comparison of microscopy, culture and molecular (PCR) methods for diagnosis of trichomoniasis, as well as on evaluation of the quality of culture media for isolation of trichomonads produced by Russian and international manufacturers. The highest sensitivity was shown for the molecular (real-time PCR) method. Microscopy of stained and wet smears demonstrated relatively high sensitivity. The culture media that are widely used in our country for Trichomonas vaginalis isolation need optimization.
Trichomonas vaginalis / Trichomonas vaginalis / diagnostics / real-time PCR / microscopy investigation / culture media
| [1] |
Гущин А. Е., Рыжих П. Г. Сравнение пределов обнаружения микроскопии, культурального посева и методов амплификации нуклеиновых кислот, используемых в лабораторной практике для выявления Trichomonas vaginalis. Клиническая дерматология и венерология. 2012; 3: 16-21. |
| [2] |
Копылов В. М., Бочкарев Е. Г., Говорун В. М. и др. Урогенитальный трихомониаз. Актуальные вопросы диагностики и лечения: пособие для врачей. 5-е изд. М.; 2005. |
| [3] |
Шипицына Е. В., Золотоверхая Е. А., Григорьев А. Н. и др. Оценка методов амплификации нуклеиновых кислот для диагностики трихомониаза. Журнал акушерства и женских болезней. 2011; 3: 73-79. |
| [4] |
Domeika M. Diagnosis of genital chlamydial infection in humans as well as in cattle. Uppsala; 1994. |
| [5] |
Domeika M., Litvinenko I., Smirnova T. et al. Laboratory diagnostics for non-viral sexually transmitted infections in St. Petersburg, Russia: current situation and hallmarks for improvements. J. Eur. Acad. Dermatol. Venereol. 2008; 22: 1094-1100. |
| [6] |
Nye M. B., Schwebke J. R., Body B. A. Comparison of APTIMA Trichomonas vaginalis transcription-mediated amplification to wet mount microscopy, culture, and polymerase chain reaction for diagnosis of trichomoniasis in men and women. Am. J. Obstet. Gynecol. 2009; 200: 188. |
| [7] |
Pillay A., Radebe F., Fehler G. et al. Comparison of a TaqMan-based real-time polymerase chain reaction with conventional tests for the detection of Trichomonas vaginalis. Sex. Transm. Infect. 2007; 83: 126-129. |
| [8] |
Schwebke J., Burgess D. Trichomoniasis. Clin. Microbiol. Rev. 2004; 17 (4): 794-803. |
| [9] |
World Health Organization. Prevalence and incidence of selected sexually transmitted infections, 2005 global estimates. Geneva: World Health Organization;2011. |
Martikaynen Z.M., Grigoryev A.N., Ryzhkova O.S., Shipitsyna Y.V., Savicheva A.M., Litvinenko I.V., Galkina A.V., Kudryavtseva N.I., Tangatarova Y.M., Lomakina O.A., Protasova Y.P., Romantseva S.Y.
/
| 〈 |
|
〉 |