Analysis of sperm DNA fragmentationin infertility patients

Evgeniya Michailovna Shilnikova; Irina Dmitrievna Fedorova; Alexander Mkrtichevich Gzgzyan

doi:10.17816/JOWD613141-147

Journal of obstetrics and women's diseases ›› 2012, Vol. 61 ›› Issue (3) : 141 -147. DOI: 10.17816/JOWD613141-147

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Analysis of sperm DNA fragmentationin infertility patients

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Abstract

Using Terminal Deoxynucleotidyl Transferase dUTP Nick End Labelling (TUNEL) technique the assessment of the frequency of the spermatozoa with fragmented DNA in the ejaculate from sperm donors and men with infertility was analyzed. The DNA fragmentation rate was higher in spermatozoa of carriers of a chromosomal structural abnormality compared with the control group. There was no correlation between the sperm DNA fragmentation rate and the parameters of semen analysis. The direct linear correlation between the frequency of the spermatozoa with fragmented DNA and vacuole sperm head was found. The DNA fragmentation rate was not correlated to the frequency of the spermatozoa with bulb, amorphous heads or spermatozoa with abnormal acrosome

Keywords

human spermatozoa / sperm DNA fragmentation / semen parameters / sperm heads

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Evgeniya Michailovna Shilnikova, Irina Dmitrievna Fedorova, Alexander Mkrtichevich Gzgzyan. Analysis of sperm DNA fragmentationin infertility patients. Journal of obstetrics and women's diseases, 2012, 61(3): 141-147 DOI:10.17816/JOWD613141-147