Effect of the extract of dry Serratula centauroides on the behavior of white rats in tests with positive reinforcement

Abstract

The effect was studied of dry leaf extract of Serratula centauroides L. on the behavior of white rats (Wistar) in tests with positive reinforcement. It was revealed that the extract of S. centauroides at doses of 50–200 mg/kg promotes in animals a decrease in the level of anxiety, adaptation to unfamiliar conditions, and as a consequence, to an increase in the volume of food intake. And also a more rapid formation of a conditioned reflex with positive reinforcement. In animals treated with S. centauroides extract, the volume of food taken in “hypophagia” test was 1.4–2.7 times higher than the control value. In the T-shaped maze, 50–80% of the animals in the experimental groups developed a conditioned reflex to positive reinforcement, while none of the animals in the control group reached the learning criterion. The S. centauroides extract showed the most pronounced effect on the behavior of animals in tests with positive reinforcement at a dose of 100 mg/kg.

Keywords

Serratula centauroides L. / dry extract / “hypophagia” test / T-shaped maze / conditioned reflex with positive reinforcement / orientation and research activity

References

[1] Olennikov DN, Kashchenko NI. Fitoehkdisteroidy nadzemnoi chasti Serratula centauroides, proizrastayushchei v Pribaikal’e. Khimija rastitel’nogo syr’ja. 2018;(2):37–44. (In Russ.)

[2] Оленников Д.Н., Кащенко Н.И. Фитоэкдистероиды надземной части Serratula centauroides, произрастающей в Прибайкалье // Химия растительного сырья. 2018. № 2. С. 37–44.

[3] Sviridov IV, Razuvaeva YaG, Shantanova LN. Influence of dry extract Serratula centauroides L. On the functional status of central nervous system. Vestnik Buryatskogo gosudarstvennogo universiteta. 2015;(12):184–188. (In Russ.)

[4] Свиридов И.В., Разуваева Я.Г., Шантанова Л.Н. Влияние экстракта сухого Serratula centauroides L. на функциональное состояние центральной нервной системы // Вестник Бурятского государственного университета. 2015. № 12. С. 184–188.

[5] Nikolaeva IG, Tsybiktarova LP, Garmaeva LL, et al. Determination of ecdysteroids in Fornicium unflorum (L.) and Serratula centauroides (L.) Raw materials by chromatography–uv spectrophotometry. Journal of Analytical Chemistry. 2017;72(8):733–741. (In Russ.) DOI: 10.1134/S1061934817080093

[6] Николаева И.Г., Цыбиктарова Л.П., Гармаева Л.Л., и др. Определение содержания экдистероидов в сырье Fornicium uniflorum и Serratula centauroides методом хроматоспектрофотометрии // Журнал аналитической химии. 2017. Т. 72, № 8. С. 733–741. DOI: 10.7868/S0044450217080047

[7] Shantanova LN, Dashiev DB, Radnaeva DB, et al. Adaptogeny v tibetskoi meditsine. Byulleten’ VSNTS SO RAN. 2008;(3):175. (In Russ.)

[8] Шантанова Л.Н., Дашиев Д.Б., Раднаева Д.Б., и др. Адаптогены в тибетской медицине // Бюллетень ВСНЦ СО РАН. 2008. № 3(61). С. 175.

[9] Sviridov IV, Khobrakova VB, Shantanova LN. The immunocorrecting effect of the dry extract from Serratula centauroides. The Siberian medical journal. 2015;(5):120–122. (In Russ.)

[10] Свиридов И.В., Хобракова В.Б., Шантанова Л.Н. Иммунокорригирующее действие экстракта Serratula centauroides // Сибирский медицинский журнал. 2015. № 5. С. 120–122.

[11] Sviridov IV. Adaptogennye svoistva ehkstrakta sukhogo Serratula centauroides [dissertation abstract]. Ulan-Ude, 2016. 23 p. (In Russ.)

[12] Свиридов И.В. Адаптогенные свойства экстракта сухого Serratula centauroides: автореф. дис. … кандидат медицинских наук. Улан-Удэ, 2016. 23 с.

[13] Garibova TL, Kraineva VA, Voronina TA. Animal models of depression. Pharmacokinetics and Pharmacodynamics. 2017;(3):14–19. (In Russ.)

[14] Воронина Т.А., Гарибова Т.Л., Крайнева В.А. Поведенческие экспериментальные модели депрессии // Фармакокинетика и фармакодинамика. 2017. № 3. С. 14–19.

[15] Mogilenko TG, Denisenko ON, Voronkov AV, et al. Izuchenie adaptogennoi i antigipoksicheskoi aktivnosti substantsii 20E, vydelennoi iz serpukhi pyatilistnoi (Serratula quinquefolia Bieb. ex Willd.), kul’tiviruemoi na Severnom Kavkaze. Modern Problems of Science and Education. Surgery. 2015;(2–3). Available at: http://science-education.ru/ru/article/view?id=23717. (In Russ.)

[16] Могиленко Т.Г., Денисенко О.Н., Воронков А.В., и др. Изучение адаптогенной и антигипоксической активности субстанции 20Е, выделенной из серпухи пятилистной (Serratula quinquefolia Bieb. ex Willd.), культивируемой на Северном Кавказе // Современные проблемы науки и образования. 2015. № 2–3. Доступ по ссылке: http://science-education.ru/ru/article/view?id=23717

[17] Pchelenko LD, Metelkina LG, Volodina SO. Adaptogennyi ehffekt ehkdisteroidsoderzhashchei fraktsii Serratula coronata L. Khimija rastitel’nogo syr’ja. 2002;(1):69–80. (In Russ.)

[18] Пчеленко Л.Д., Метелкина Л.Г., Володина С.О. Адаптогенный эффект экдистероидсодержащей фракции Serratula coronata L. // Химия растительного сырья. 2002. № 1. С. 69–80.

[19] Bajguz A, Bąkała I, Talarek M. Chapter 5 – Ecdysteroids in Plants and their Pharmacological Effects in Vertebrates and Humans. Studies in Natural Products Chemistry. 2015;45:121–145. DOI: 10.1016/B978-0-444-63473-3.00005-8

[20] Bajguz A., Bąkała I., Talarek M. Chapter 5 – Ecdysteroids in Plants and their Pharmacological Effects in Vertebrates and Humans // Studies in Natural Products Chemistry. 2015. Vol. 45. P. 121–145. DOI: 10.1016/B978-0-444-63473-3.00005-8

[21] Volodin VV, Sidorova YuS, Mazo VK. 20-hydroxyecdysone – plant adaptogen: an anabolic effect, possible use in sports nutrition. Problems of nutrition. 2013;82(6):24–30. (In Russ.)

[22] Володин В.В., Сидорова Ю.С., Мазо В.К. 20-гидроксиэкдизон — растительный адаптоген: анаболическое действие, возможное использование в спортивном питании // Вопросы питания. 2013. Т. 82, № 6. С. 24–30.

[23] Cahlikova L, Macakova K, Chlebek J, et al. Ecdysterone and its Activity on some Degenerative Diseases. Natural Product Communications. 2011;6(5):707–718. DOI: 10.1177/1934578X1100600527

[24] Cahlikova L., Macakova K., Chlebek J., et al. Ecdysterone and its Activity on some Degenerative Diseases // Natural Product Communications. 2011. Vol. 6. No. 5. P. 707–718. DOI: 10.1177/1934578X1100600527

[25] Mamadalieva NZ, Böhmdorfer S, Zengin G, et al. Phytochemical and biological activities of Silene viridiflora extractives. Development and validation of a HPTLC method for quantification of 20-hydroxyecdysone. Industrial Crops and Products. 2019;129:542–548. DOI: 10.1016/j.indcrop.2018.12.041

[26] Mamadalieva N.Z., Böhmdorfer S., Zengin G., et al. Phytochemical and biological activities of Silene viridiflora extractives. Development and validation of a HPTLC method for quantification of 20-hydroxyecdysone // Industrial Crops and Products. 2019. Vol. 129. P. 542–548. DOI: 10.1016/j.indcrop.2018.12.041

[27] Lafont R, Dinan L. Current state of the problem of the practical use of ecdysteroids for mammals and humans. Vestnik Insituta biologii Komi NC UrO RAN. 2005;(3):2–18. (In Russ.)

[28] Лафон Р., Дайнан Л. Современное состояние проблемы практического использования экдистероидов для млекопитающих и человека // Вестник Института биологии Коми НЦ УрО РАН. 2005. № 3. С. 2–18.

[29] Galdino PM, Nascimento MМ, Florentino IF. The anxiolytic-like effect of an essential oil derived from Spiranthera odoratissima A. St. Hil. leaves and its major component, beta-caryophyllene, in male mice. Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2012;38(2):276–284. DOI: 10.1016/j.pnpbp.2012.04.012.

[30] Galdino P.M., Nascimento M.М., Florentino I.F. The anxiolytic-like effect of an essential oil derived from Spiranthera odoratissima A. St. Hil. leaves and its major component, beta-caryophyllene, in male mice // Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2012. Vol. 38. No. 2. P. 276–284. DOI: 10.1016/j.pnpbp.2012.04.012.

RIGHTS & PERMISSIONS

Razuvaeva Y.G., Markova K.V., Toropova A.A., Olennikov D.N.