Effect of glyprolins on white blood cell parameters and phagocytic activity of neutrophils in conditions of experimental hyperthyroidism

Abstract

This study investigated the effects of glyproline neuropeptide compounds (selank and Pro-Gly-Pro) on the white blood cell count and phagocytic activity of neutrophils in 40 nonlinear white male rats aged 6–8 months with experimental hyperthyroidism. Experimental hyperthyroidism was simulated by intragastric administration of L-thyroxine sodium salt pentahydrate at a dose of 150 µg/kg/day for 21 days. All animals were equally divided into four groups: healthy rats (control), rats treated with L-thyroxine sodium pentahydrate (hyperthyroidism), rats treated with selank, and rats treated with Pro-Gly-Pro at doses of 200 µg/kg/day intraperitoneally for 21 days, starting one day after the last administration of L-thyroxine sodium pentahydrate. After the animals were removed from the experiment, the white blood cell count and the percentage of lymphocytes, stick, and segmentonuclear neutrophils were calculated, and the phagocytic activity of neutrophils was evaluated. In the case of experimental hyperthyroidism, neutrophilic leukocytosis with a shift to the right, lymphopenia, and decreased phagocytic activity of neutrophils were observed. Glyproline neuropeptides contributed to the correction of observed changes in white blood cell indices and phagocytic processes, which indicates the immunocorrigating effect of the test compounds. Thus, glyproline neuropeptides demonstrated pronounced immunotropic activity, which manifested in the correction of changes arising from the leukocyte count and phagocytosis processes. However, further detailed study of the pharmacological effects of neuropeptide agents on experimental hyperthyroidism is necessary.

Keywords

glyprolins / neuropeptides / selank / experimental hyperthyroidism / immunotropic activity / leukocytes / phagocytic number / phagocytic index

References

[1] Taylor PN, Albrecht D, Scholz A, et al. Global epidemiology of hyperthyroidism and hypothyroidism. Nat Rev Endocrinol. 2018;14(5):301–316. DOI: 10.1038/nrendo.2018.18

[2] Taylor P.N., Albrecht D., Scholz A., et al. Global epidemiology of hyperthyroidism and hypothyroidism // Nat Rev Endocrinol. 2018. Vol. 14. No. 5. Р. 301–316. DOI: 10.1038/nrendo.2018.18

[3] Doubleday AR, Sippel RS. Hyperthyroidism. Gland Surg. 2020;9(1):124–135. DOI: 10.21037/gs.2019.11.01

[4] Doubleday A.R., Sippel R.S. Hyperthyroidism // Gland Surg. 2020. Vol. 9. No. 1. Р. 124–135. DOI: 10.21037/gs.2019.11.01

[5] Lane LC, Cheetham TD, Perros P, Pearce SHS. New Therapeutic Horizons for Graves’ Hyperthyroidism. Endocr Rev. 2020;41(6): 873–884. DOI: 10.1210/endrev/bnaa022

[6] Lane L.C., Cheetham T.D., Perros P., Pearce S.H.S. New Therapeutic Horizons for Graves’ Hyperthyroidism // Endocr Rev. 2020. Vol. 41. No. 6. Р. 873–884. DOI: 10.1210/endrev/bnaa022

[7] Rivas AM, Pena C, Kopel J, et al. Hypertension and Hyperthyroidism: Association and Pathogenesis. Am J Med Sci. 2021;361(1):3–7. DOI: 10.1016/j.amjms.2020.08.012

[8] Rivas A.M., Pena C., Kopel J., et al. Hypertension and Hyperthyroidism: Association and Pathogenesis // Am J Med Sci. 2021. Vol. 361. No. 1. Р. 3–7. DOI: 10.1016/j.amjms.2020.08.012

[9] Tsai K, Leung AM. Subclinical Hyperthyroidism: A Review of the Clinical Literature. Endocr Pract. 2021;27(3):254–260. DOI: 10.1016/j.eprac.2021.02.002

[10] Tsai K., Leung A.M. Subclinical Hyperthyroidism: A Review of the Clinical Literature // Endocr Pract. 2021. Vol. 27. No. 3. Р. 254–260. DOI: 10.1016/j.eprac.2021.02.002

[11] Noda M. Thyroid Hormone in the CNS: Contribution of Neuron-Glia Interaction. Vitam Horm. 2018;106:313–331. DOI: 10.1016/bs.vh.2017.05.005

[12] Noda M. Thyroid Hormone in the CNS: Contribution of Neuron-Glia Interaction // Vitam Horm. 2018. Vol. 106. Р. 313–331. DOI: 10.1016/bs.vh.2017.05.005

[13] Chenchak VA. Features of the action of thyroxin on the immune system. 2017;(3):38–38. (In Russ.).

[14] Ченчак В.А. Особенности действия тироксина на иммунную систему // Международный студенческий научный вестник. 2017. № 3. С. 38–38.

[15] Zenkov AL. O vliyanii tiroksina na immunokompetentnye kletki. International Conference on Chemical, Biological and Health Sciences. Pisa, 2017. P. 117–128. (In Russ.).

[16] Зенков А.Л. О влиянии тироксина на иммунокомпетентные клетки // International Conference on Chemical, Biological and Health Sciences. Pisa, 2017. С. 117–128.

[17] Carr R, Frings S. Neuropeptides in sensory signal processing. Cell and Tissue Res. 2019;375(1):217–225. DOI: 10.1007/s00441-018-2946-3

[18] Carr R., Frings S. Neuropeptides in sensory signal processing // Cell and Tissue Res. 2019. Vol. 375. No. 1. Р. 217–225. DOI: 10.1007/s00441-018-2946-3

[19] Yasenyavskaya AL, Samotrueva MA, Myasoedov NF, Andreeva LA. Influence of semax on the level of interleukin-1βin the conditions of “social” stress. Medical academic journal. 2019;9(1S): 192–194. (In Russ.). DOI: 10.17816/MAJ191S1192-194

[20] Ясенявская А.Л., Самотруева М.А., Мясоедов Н.Ф., Андреева Л.А. Влияние семакса на уровень интерлейкина-١β в условиях «социального» стресса // Медицинский академический журнал. 2019. Т. 9, № 1S. С. 192–194. DOI: 10.17816/MAJ191S1192-194

[21] Vyunova TV, Andreeva LA, Shevchenko KV, Myasoedov NF. An integrated approach to study the molecular aspects of regulatory peptides biological mechanism. J Label Compd Radiopharm. 2019;62(12):812–822. DOI: 10.1002/jlcr.3785

[22] Vyunova T.V., Andreeva L.A., Shevchenko K.V., Myasoedov N.F. An integrated approach to study the molecular aspects of regulatory peptides biological mechanism // J Label Compd Radiopharm. 2019. Vol. 62. No. 12. Р. 812–822. DOI: 10.1002/jlcr.3785

[23] Thiele TE. Neuropeptides and Addiction: An Introduction International. Rev Neurobiol. 2017;136:1–3. DOI: 10.1016/bs.irn.2017.07.001

[24] Thiele T.E. Neuropeptides and Addiction: An Introduction International // Rev Neurobiol. 2017. Vol. 136. Р. 1–3. DOI: 10.1016/bs.irn.2017.07.001

[25] Sergalieva MU, Tsibizova AA, Abdulkadyrova EI. The effect of selank and pro-gly-proon behavioral responses white rats in the Porsolt test in the conditions of experimental hyperthyroidism. Astrakhan medical journal. 2021;16(2):53–61. (In Russ.). DOI: 10.17021/2021.16.2.53.61

[26] Сергалиева М.У., Цибизова А.А., Абдулкадырова Э.И., и др. Влияние селанка и Pro-Gly-Pro на поведенческие реакции белых крыс в тесте «Порсолт» в условиях экспериментального гипертиреоза // Астраханский медицинский журнал. ٢٠٢١. Т. ١٦, № 2. С. 53–61. DOI: 10.17021/2021.16.2.53.61

[27] Shcherba VV, Korda MM. The state of the nitrogen (II) oxidesystem in rats with periodontitis on the background of hyper- and hypothyroidism. Medical and Clinical Chemistry. 2018;2(1):143. (In Ukraine). DOI: 10.11603/mcch.2410-681X.2018.v0.i1.8844

[28] Щерба В.В., Корда М.М. Фагоцитарна і метаболічна активність нейтрофілів крові у щурів з пародонтитом на фоні гіпер-та гіпотиреозу // Вісник проблем біології і медицини. 2018. Т. 2, № 1. С. 143. DOI: 10.11603/mcch.2410-681X.2018.v0.i1.8844

RIGHTS & PERMISSIONS

Sergalieva M.U., Tsybizova A.A., Andreeva L.A., Myasoedov N.F., Bashkina O.A., Samotrueva M.A.