Experimental evaluation of the radiomodifying efficiency of low-intensity electromagnetic radiation in acute x-ray irradiation of mice

Abstract

It was found that acute exposure to ionizing radiation at doses of 7.5 and 8 Gy leads to the development of bone marrow syndrome of acute radiation sickness in mice. On the 7th day after exposure at doses of 7.5 Gy and 8 Gy, the mortality rate of animals was 66.7%, on the 10th day 83.3 and 86.7%, and by the 14th day it reached 91.7 and 100%, respectively. Prophylactic exposure to electromagnetic radiation from a helium-neon laser modulated with preparations with the tissue of the hypothalamic structures of the brain, spleen and bone marrow of a newborn mouse (P1-4) before X-ray irradiation at doses of 7.5 and 8 Gy contributed to a decrease in the mortality of animals from acute radiation sickness during the first 14 days, which was 28.6 and 50%, respectively. However, with this method of protective action, by the 22nd day after the radiation damage of 7.5 Gy, the mortality rate reached 64.3%, and 8 Gy-90%. On the contrary, with a therapeutic and preventive method of exposure to electromagnetic radiation by a helium-neon laser modulated by drugs with tissue from hypothalamic structures of the brain, spleen and bone marrow of a newborn mouse (R1-4) after a radiation lesion at a dose of 7.5 Gy, the mortality on the 25th day was 23.3%, and 8 Gy — 30% and remained at this level for more than 30 days. We believe that the increase in the resistance of mice to ionizing radiation and the different nature of the course of acute radiation sickness with preventive and therapeutic methods of exposure is due to several factors. On the one hand, it is the realization of antihypoxic, antioxidant effects when exposed to electromagnetic radiation modulated by drugs with tissue from hypothalamic structures of the brain, spleen and bone marrow of a newborn mouse. On the other hand, the effect of electromagnetic radiation modulated by the preparation of the bone marrow and spleen of a newborn mouse has a cytoprotective effect on the bone marrow cells of mice with acute radiation sickness. It is also possible that this effect contributes to adequate neuroimmune regulation in the development of acute radiation sickness in mice.

Keywords

ionizing radiation / acute radiation sickness / resistance / superweak effects / electromagnetic radiation / laser radiation / biostructures / radiomodifying action

References

[1] Nikiforov AS, Ivanov IM, Sventickaya AM, et al. Modelirovanie ostrogo luchevogo kostnomozgovogo sindroma v eksperimente na myshah. Mediko-biologicheskie i social’no-psihologicheskie problemy bezopasnosti v chrezvychajnyh situaciyah. 2017;(4):66–71. (In Russ.).

[2] Никифоров А.С., Иванов И.М., Свентицкая А.М., и др. Моделирование острого лучевого костномозгового синдрома в эксперименте на мышах // Медико-биологические и социально-психологические проблемы безопасности в чрезвычайных ситуациях. 2017. № 4. С. 66–71.

[3] Kucenko SA, Butomo NV, Grebenyuk AN, et al. Voennaya toksikologiya, radiobiologiya i medicinskaya zashchita. St. Petersburg: Foliant; 2004. (In Russ.).

[4] Куценко С.А., Бутомо Н.В., Гребенюк А.Н., и др. Военная токсикология, радиобиология и медицинская защита. СПб.: Фолиант, 2004.

[5] Ovchinnikov YuV, Halimov YuSh, editors. Voenno-polevaya terapiya. St. Petersburg: ELBI-SPb; 2016. (In Russ.).

[6] Военно-полевая терапия / под ред. Овчинникова Ю.В., Халимова Ю.Ш. СПб.: ЭЛБИ-СПб, 2016.

[7] Grebenyuk AN, Legeza VI. Perspektivy ispol’zovaniya radioprotektorov dlya povysheniya medicinskoj protivoradiacionnoj zashchity Vooruzhennyh sil. Voenno-medicinskij zhurnal. 2013;334(7):46–50. (In Russ.).

[8] Гребенюк А.Н., Легеза В.И. Перспективы использования радиопротекторов для повышения медицинской противорадиационной защиты Вооруженных сил // Военно-медицинский журнал. 2013. Т. 334, № 7. С. 46–50.

[9] Kokaya AA, Mironov AA, Kokaya NG, et al. Specifichnost’ dejstviya elektromagnitnogo izlucheniya, preobrazovannogo razlichnymi biostrukturami. Vestnik Rossijskoj Voenno-Medicinskoj akademii. 2012;(40):163–168. (In Russ.).

[10] Кокая А.А., Миронов А.А., Кокая Н.Г., и др. Специфичность действия электромагнитного излучения, преобразованного различными биоструктурами // Вестник Российской военно-медицинской академии. 2012. № 40. С. 163–168.

[11] Kokaya AA, Vedunova MV, Mitroshina EV, et al. CHuvstvitel’nost’ nejronov k nizkointensivnomu elektromagnitnomu izlucheniyu pri toksicheskom dejstvii gidrazinov. Vestnik Rossijskoj Voenno-Medicinskoj akademii. 2013;(42):109–115. (In Russ.).

[12] Кокая А.А., Ведунова М.В., Митрошина Е.В., и др. Чувствительность нейронов к низкоинтенсивному электромагнитному излучению при токсическом действии гидразинов // Вестник Российской военно-медицинской академии. 2013. № 42. С. 109–115.

[13] Kokaya AA, Vedunova MV, Mitroshina EV, et al. Ustojchivost’ nejronov k normobaricheskoj gipoksii in vitro pri vozdejstvii nizkointensivnym elektromagnitnym izlucheniem. Vestnik Rossijskoj Voenno-Medicinskoj Akademii. 2014;(45):127–131. (In Russ.).

[14] Кокая А.А., Ведунова М.В., Митрошина Е.В., и др. Устойчивость нейронов к нормобарической гипоксии in vitro при воздействии низкоинтенсивным электромагнитным излучением // Вестник Российской военно-медицинской академии. 2014. № 45. С. 127–131.

[15] Kokaya NG, Kokaya AA, Muhina IV. Vliyanie modulirovannogo biostrukturami elektromagnitnogo izlucheniya na otdalennye adaptacionnye strukturnye perestrojki kletok pecheni u krys s eksperimental’nym saharnym diabetom. Vestnik novyh medicinskih tekhnologij 2011;(3):123–126. (In Russ.).

[16] Кокая Н.Г., Кокая А.А., Мухина И.В. Влияние модулированного биоструктурами электромагнитного излучения на отдаленные адаптационные структурные перестройки клеток печени у крыс с экспериментальным сахарным диабетом // Вестник новых медицинских технологий. 2011. № 3. С. 123–126.

[17] Buchachenko AL. Radioizluchenie i drugie magnitnye effekty v himicheskih reakciyah. Мoscow: Znanie; 2007. (In Russ.).

[18] Бучаченко А.Л. Радиоизлучение и другие магнитные эффекты в химических реакциях. М.: Знание, 2007.

[19] Buchachenko AL. Novaya izotopiya v himii i biohimii. Moscow: Nauka; 2007. (In Russ.).

[20] Бучаченко А.Л. Новая изотопия в химии и биохимии. М.: Наука, 2007.

RIGHTS & PERMISSIONS

Kokaya G.N., Kokaya A.A., Kozyakov V.P., Zavirsky A.V., Zatsepin V.V., Basharin V.A., Tsygan V.N., Mavrenkov E.M.