Isolation of biologically active substances from plant objects in military field technology by the example of Urtica dioica L.

E. F. Stepanova; A. G. Kuregyan; S. V. Pechinsky; Yu. Y. Zhidkova

doi:10.17816/brmma623041

Bulletin of the Russian Military Medical Academy ›› 2017, Vol. 19 ›› Issue (3) : 134 -139. DOI: 10.17816/brmma623041

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Isolation of biologically active substances from plant objects in military field technology by the example of Urtica dioica L.

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Abstract

The issue of rational use of medicinal plant raw materials in the field during wartime is considered. The role of the military field technology of medicines is noted, both in the historical and in the modern aspect. An approach to the selection of raw materials based on the structure of morbidity in wartime and the pharmacological effect of medicinal plant raw materials is outlined. Bleeding and blood loss are highlighted as prevalent in combat pathology. On the example of a nettle, a method for biologically active substances isolation in military field technology is described, based on the theoretical hypothesis of the authors. The chemical composition of nettle leaves is given. The content of carotenoids (up to 0,05% in total) was classified due to their kind. The dominant compound is lutein. The materials and methods of the study are described in detail. The effectiveness of petroleum ether and ethyl alcohol 95% in a role of extractants is proved. The result was achieved by successive treatment of the feedstock with several extractants in the decreasing order of polarity. A method for separating carotenoids and chlorophylls by the method of liquid extraction is proposed. Isolation of carotenes, xanthophylls and chlorophylls from the total lipophilic extract, not contaminated by other groups of biologically active substances, is described.

Keywords

military field technology / stinging nettle / extraction / vitamin K / carotenoids / lutein / chlorophylls / carotene / xanthophylls

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E. F. Stepanova, A. G. Kuregyan, S. V. Pechinsky, Yu. Y. Zhidkova. Isolation of biologically active substances from plant objects in military field technology by the example of Urtica dioica L.. Bulletin of the Russian Military Medical Academy, 2017, 19(3): 134-139 DOI:10.17816/brmma623041

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