Using the Paramecium caudatum test object to determine acute toxicity of physiologically active substances
V. A. Andreev , E. Yu. Andreeva , L. P. Erdniyev , Ya. A. Stepanov , A. Yu. Mikshta , I. V. Mokshanov , I. A. Yermolayeva , N. V. Stepanova , V. Ya. Apchel
Bulletin of the Russian Military Medical Academy ›› 2019, Vol. 21 ›› Issue (2) : 110 -113.
Using the Paramecium caudatum test object to determine acute toxicity of physiologically active substances
The advantage of biotesting in comparison with the physicochemical analysis of the use of Paramecium caudatum for toxicological studies has been shown. Physical and chemical analysis often fails to detect unstable compounds. In addition, these tools are not possible to determine the ultra-low concentration of chemicals. In addition to the traditional use of laboratory animals, representatives of protozoa have recently been used. In connection with this, a test object of the Simplest type was chosen and justified. The advantages of using the simplest compared with the use of laboratory animals. The various models used to test toxic substances are described. Their advantages and disadvantages are noted. The main advantages of second-order models using alternative models (various hydrobionts, bacteria, cell cultures, etc.) are described. The relevance of using the culture of cells of the ciliate Paramecium caudatum for toxicological studies has been shown. The methodological aspects of the biotesting of organic solvents using the proposed test object are presented. A unidirectional change in the toxicometric parameters of organic solvents for two types of biological objects: a white mouse and a culture of infusoria Paramecium caudatum were revealed. The possibility of using Paramecium caudatum for toxicological studies has been shown. A correlation was noted between the indicators of the general toxicity of organic dyes and those of white mice.
ciliate Paramecium caudatum / biotesting / organic solvent / toxicometry / test objects / acute toxicity / biological models / culturing of infusoria / Lozina-Lozinsky medium / laboratory animals
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Andreev V.A., Andreeva E.Y., Erdniyev L.P., Stepanov Y.A., Mikshta A.Y., Mokshanov I.V., Yermolayeva I.A., Stepanova N.V., Apchel V.Y.
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