Experimental substantiation of the possibility of using in ophthalmosurgery two-component fibrin glue, prepared according to our own methods

Abstract

As an alternative to fixing tissues with sutures in world surgery, the method of their adhesion using fibrin-based adhesive compositions is finding increasing use. However, they are so far inaccessible to surgeons, due to the lack of certification in our country. The cyanoacrylate glue used previously by domestic ophthalmologists had insufficiently pronounced adhesive properties and some histotoxicity and is practically not used at present. Experimentally substantiates the possibility of applying in ophthalmology a modified method of manufacturing a two-component fibrin glue. Two series of experiments were carried out to test the original method of preparing the components of fibrin glue. A preliminary study of its adhesive properties was carried out on the eyes of three rabbits (6 eyes) of the Chinchilla breed by gluing the amniotic membrane on the corneal stroma after partial surface keratectomy. Another six rabbits of the Chinchilla breed (12 eyes) carried out transplantation of the amniotic membrane on the corneal stroma: for the right eyes, interrupted sutures (group A), and for the left eyes – fibrin glue (group B). Postoperatively, the healing process was monitored dynamically using biomicroscopy and photo fixation on the 1st, 3rd, 5th, 7th, 10th, 15th, 30th, 45th and 90th day. Additionally, on the 30th day optical coherent tomography of the corneas of experimental animals was performed. The qualitative and quantitative composition of the components has been determined, which ensures the manufacture of fibrin glue, as well as its application in vivo by fixing the biomembrane (amnion) to the de-epithelialized cornea. The proposed method of preparation of fibrin glue provides reliable adhesion of the amniotic membrane to the stroma of the cornea and reduces the time of the operation. The method creates conditions for an earlier onset of epithelialization and contributes to the preservation of the transparency of the cornea compared with suture fixation.

Keywords

fibrin glue / adhesion / amniotic membrane / suture fixation / cornea / regeneration / ophthalmosurgery / reconstruction / eye surface

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RIGHTS & PERMISSIONS

Kuznetsova A.Yu., Gavrilyuk I.O., Churashov S.V., Kulikov A.N., Chernysh V.F., Gavrilyuk V.N., Muraveva O.A., Danilichev V.F.