Hair research for the diagnosis of psychoactive substance use

Abstract

The possibility of using hair as one of the objects of research for the diagnosis not only the fact of use of psychoactive substances but also identification the statute of limitations and duration of using toxic substances is considered. These results are important not only in medical practice to provide doctors with a compфlete picture of remission in patients (F10–F19), but also in judicial practice to resolve social issues, for example, custody issues. A model of long-term use of substances (phenobarbital and diphenhydramine) was developed on laboratory animals – guinea pigs of white, black and red natural colour. Enzymatic hydrolysis techniques (chymotrypsin, hemopexin and papain) was developed to isolate psychoactive substances from the hair staff of laboratory animals. phenobarbital and diphenhydramine were detected in animal hair 28 days after the substance was discontinued. Phenobarbital was detected in an amount of from 18,91 to 19,85 ng/mg, diphenhydramine from 5,71 to 7,04 ng/mg. The developed methods of enzymatic hydrolysis were tested on expert material (hair) of the examined persons collected according to the recommendations of the Order of the Ministry of Health of the Russian Federation № 40 of January 27, 2006. In total, 118 hair samples were analyzed during the study, 14 samples showed a positive analysis for the presence of psychoactive and other substances: methamphetamine, methylecgonine, cocaine, amphetamine, formethorex, pirogerone, methadone, tetrahydrocannabinol, phenobarbital, tropicamide, diphenhydramine, salbutamol, nicotine. Developed methods of isolation allow screening search to detect psychoactive substances for the diagnosis of their use.

Keywords

hair / psychoactive substances / enzymatic hydrolysis / acid hydrolysis / alkaline hydrolysis / examined person / phenobarbital / diphenhydramine / hair of laboratory animals

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RIGHTS & PERMISSIONS

Slustovskaya Yu.V., Krysko M.V., Strelova O.Yu., Kuklin V.N.