Levels of prooxidant-antioxidant balance indicators in children with the mixed form of chronic glomerulonephritis

Abstract

The condition of lipid peroxidation and antioxidant system in 88 children with the mixed form of chronic glomerulonephritis is studied at the age of 13,84±1,75. The control group consisted of 30 healthy kids. We use clinical, laboratory and instrumental study methods. The rate of lipid peroxidation was assessed by levels of diene conjugates and malondialdehyde. The condition of an antioxidant system was determined by catalase, reduced glutathione, glutathione peroxidase and glutathione reductase. A significant increase in diene conjugates in erythrocytes was detected by 88.3% (p<0.01) and malonic dialdehyde in blood plasma and erythrocytes by 67.5 and 83.4% (p <0.01), respectively. The recrudescence of the pathological process increased the accumulation of primary and secondary products of lipid peroxidation. The concentration of catalase in plasma and erythrocytes on the average was lower than in the control one by 19.4 and 26.2% (p<0.05), respectively; reduced glutathione by 17.1%, in erythrocytes - by 41.9% (p <0.05). A reliably low level of glutathione peroxidase was detected in blood plasma (20.6%) and in erythrocytes (44.9%), the level of glutathione reductase was reduced by 35.7% and 50.0% (p <0.05) in plasma and in erythrocytes respectively. Accumulation of products of lipid peroxidation and a mismatch in the activity of antioxidant enzymes indicates the development of chronic antioxidant stress and the weakening of the adaptive potential in children with a mixed form of chronic glomerulonephritis. In general, the progression of the mixed form of chronic glomerulonephritis is associated with shifts in the state of prooxidant-antioxidant equilibrium.

Keywords

chronic glomerulonephritis / mixed form / kids / children / lipid peroxidation / antioxidant system

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