Protective role of silver nanoparticles in influenza infection

Abstract

BACKGROUND: The present study assesses copper metabolism of the host organism as a target of antiviral strategy, basing on the “virocell” concept. This concept suggests that the targets for suppressing viral reproduction can be found in the host’s metabolism.

AIM: Evaluation of the effect of copper status indicators on influenza infection in mice.

MATERIALS AND METHODS: Silver nanoparticles (AgNPs) were used as a specific active agent because they reduce the level of holo-ceruloplasmin, the main extracellular cuproenzyme. The mouse model of influenza virus A infection was used with two doses: 1 LD₅₀ and 10 LD₅₀. The following treatment regimens were used: mice were pretreated four days before infection and then every day during infection development until the end of the experiment (day 14).

RESULTS: The mice treated with AgNPs demonstrated significantly lower mortality, the protection index reached 60–70% at the end of the experiment, and mean lifespan was prolonged. In addition, the treatment of the animals with AgNPs resulted in normalization of the weight dynamics. Despite the amelioration of the infection, AgNPs treatment did not influence influenza virus replication.

CONCLUSIONS: This study provides support for the view that silver nanoparticles could be used as protection against influenza.

Keywords

ceruloplasmin / copper status / silver nanoparticles / influenza / influenza virus replication / prophylaxis and treatment

References

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RIGHTS & PERMISSIONS

Al Farroukh M., Skomorokhova E.A., Magazenkova D.N., Kiseleva I.V.