Changes in the activity of amylase, alkaline phosphatase and lipase in placenta and umbilical cord blood in women in labor with gestational diabetes mellitus

Abstract

BACKGROUND: The prevalence of gestational diabetes mellitus in the world is increasing every year. However, there is a small number of studies on changes in enzymes of various classes in blood serum in gestational diabetes mellitus, and there are conflicting results regarding the concentration of individual enzymes.

AIM: To study the activity of amylase, alkaline phosphatase and lipase in placenta and umbilical cord blood in women in labor with gestational diabetes mellitus in comparison with women without this pathology.

METHODS: Cord blood and placenta sections were obtained from women with (n = 20) and without (n = 62) gestational diabetes mellitus during labor in the presence of informed consent. Alkaline phosphatase and lipase activity was estimated by kinetic colorimetric method, amylase activity by amyloclastic method according to Caraway.

RESULTS: It has been shown that in pregnant women with gestational diabetes mellitus who are on diet therapy, significant differences in the activity of amylase and alkaline phosphatase are present in the homogenate of the placenta and umbilical cord blood compared with pregnant women without gestational diabetes mellitus. In pregnant women without gestational diabetes mellitus and women on insulin therapy, no significant differences were found in the studied fluids according to these enzymes. There were no significant differences in the lipolytic activity of umbilical cord blood and placental homogenate between the groups of women studied.

CONCLUSION: The study showed that gestational diabetes mellitus in women leads to significant changes in the activity of enzymes in the homogenate of the placenta and umbilical cord blood.

Keywords

gestational diabetes mellitus / enzymes / pregnancy / placenta / umbilical cord blood

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