Vitral assessment of mast cells degranulation mediated by IgG antibodies

Abstract

BACKGROUND: Data on the potential for mast cell activation and degranulation under these IgG-containing immune complexes indicate the presence of another innovative pathway for mast cell activation and help explain the severe infection following vaccination.

AIM: The aim of the study was to evaluate the possibility of activation and degranulation of mast cells of peritoneal exudate in mice by binding Fcγ receptors.

MATERIALS AND METHODS: Influenza viruses A/Vietnam/1194/2004(H5N1) NIBRG-14 and A/New York/61/ 2015(H1N1)pdm09 were used in the work. Cells of the peritoneal exudate of CBA mice, containing an average of 7–10% mast cells, were used as a source of mast cells. The degranulation of mast cells 40 min after the introduction of IgG-containing immune complexes into cultures was assessed by the release of histamine into culture attachments. The histamine level was determined fluorimetrically after the formation of its complexes with orthophthalic aldehyde, and the histamine concentration was expressed in ng/ml.

RESULTS: In response to the binding of Fcγ receptors, a dose-dependent release of histamine from the mast cells occurs. Histamine production was noted both during the introduction of model immune complexes formed by thermally aggregated IgG, and during the formation of complexes including IgG and influenza viruses of different strains. A higher level of histamine secretion was noted during the formation of IgG complexes with the H5N1 influenza virus. The level of histamine mast cells production during Fcγ receptor binding was comparable to the response to Fcε receptor binding.

CONCLUSIONS: Binding of immune complexes containing IgG class immunoglobulins to receptors on the surface of peritoneal exudate mast cells leads to their activation and degranulation, which is accompanied by dose-dependent histamine secretion, the level of which also depends on the strain of influenza virus in the complex.

Keywords

mast cells / histamine / IgG antibodies / influenza viruses of H5N1 and H1N1 strains

References

[1] Agier J, Różalska S, Wiktorska M, et al. The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2. Sci Rep. 2018;8(1):11750. doi: 10.1038/s41598-018-30289-w

[2] Agier J., Różalska S., Wiktorska M., et al. The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2 // Sci Rep. 2018. Vol. 8, N 1. P. 11750. doi: 10.1038/s41598-018-30289-w

[3] Prussin C, Metcalfe DD. 5. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol. 2006;117(2 Suppl Mini-Primer):S450–S456. doi: 10.1016/j.jaci.2005.11.016

[4] Prussin C., Metcalfe D.D. 5. IgE, mast cells, basophils, and eosinophils // J Allergy Clin Immunol. 2006. Vol. 117, N 2 Suppl Mini-Primer. P. S450–S456. doi: 10.1016/j.jaci.2005.11.016

[5] Hu Y, Jin Y, Han D, et al. Mast cell-induced lung injury in mice infected with H5N1 influenza virus. J Virol. 2012;86(6):3347–3356. doi: 10.1128/JVI.06053-11

[6] Hu Y., Jin Y., Han D., et al. Mast cell-induced lung injury in mice infected with H5N1 influenza virus // J Virol. 2012. Vol. 86, N 6. P. 3347–3356. doi: 10.1128/JVI.06053-11

[7] Zarnegar B, Westin A, Evangelidou S, Hallgren J. Innate immunity induces the accumulation of lung mast cells during influenza infection. Front Immunol. 2018;9:2288. doi: 10.3389/fimmu.2018.02288

[8] Zarnegar B., Westin A., Evangelidou S., Hallgren J. Innate immunity induces the accumulation of lung mast cells during influenza infection // Front Immunol. 2018. Vol. 9. P. 2288. doi: 10.3389/fimmu.2018.02288

[9] Liu B, Meng D, Wei T, et al. Apoptosis and pro-inflammatory cytokine response of mast cells induced by influenza A viruses. PLoS One. 2014;9(6):e100109. doi: 10.1371/journal.pone.0100109

[10] Liu B., Meng D., Wei T., et al. Apoptosis and pro-inflammatory cytokine response of mast cells induced by influenza A viruses // PLoS One. 2014. Vol. 9, N 6. P. e100109. doi: 10.1371/journal.pone.0100109

[11] Wu H, Zhang S, Huo C, et al. iTRAQ-based proteomic and bioinformatic characterization of human mast cells upon infection by the influenza A virus strains H1N1 and H5N1. FEBS Lett. 2019;593(18):2612–2627. doi: 10.1002/1873-3468.13523

[12] Wu H., Zhang S., Huo C., et al. iTRAQ-based proteomic and bioinformatic characterization of human mast cells upon infection by the influenza A virus strains H1N1 and H5N1 // FEBS Lett. 2019. Vol. 593, N 18. P. 2612–2627. doi: 10.1002/1873-3468.13523

[13] Ricke DO. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. Front Immunol. 2021;12:640093. doi: 10.3389/fimmu.2021.640093

[14] Ricke D.O. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies // Front Immunol. 2021. Vol. 12. P. 640093. doi: 10.3389/fimmu.2021.640093

[15] Wong JP, Christopher ME, Viswanathan S, et al. Activation of toll-like receptor signaling pathway for protection against influenza virus infection. Vaccine. 2009;27(25–26):3481–3483. doi: 10.1016/j.vaccine.2009.01.048

[16] Wong J.P., Christopher M.E., Viswanathan S., et al. Activation of toll-like receptor signaling pathway for protection against influenza virus infection // Vaccine. 2009. Vol. 27, N 25–26. P. 3481–3483. doi: 10.1016/j.vaccine.2009.01.048

[17] Hu Y, Jin Y, Han D, et al. Mast cell-induced lung injury in mice infected with H5N1 influenza virus. J Virol. 2012;86(6):3347–3356. doi: 10.1128/JVI.06053-11

[18] Hu Y., Jin Y., Han D., et al. Mast cell-induced lung injury in mice infected with H5N1 influenza virus // J Virol. 2012. Vol. 86, N 6. P. 3347–3356. doi: 10.1128/JVI.06053-11

[19] Pelaia G, Vatrella A, Gallelli L, et al. Respiratory infections and asthma. Respir Med. 2006;100(5):775–784. doi: 10.1016/j.rmed.2005.08.025

[20] Pelaia G., Vatrella A., Gallelli L., et al. Respiratory infections and asthma // Respir Med. 2006. Vol. 100, N 5. P. 775–784. doi: 10.1016/j.rmed.2005.08.025

[21] Mamontov A, Losev I, Korzhevskii D, et al. Study of antibody-dependent reactions of mast cells in vitro and in a model of severe influenza infection in mice. Front Immunol. 2021;12:689436. doi: 10.3389/fimmu.2021.689436

[22] Mamontov A., Losev I., Korzhevskii D., et al. Study of antibody-dependent reactions of mast cells in vitro and in a model of severe influenza infection in mice // Front Immunol. 2021. Vol. 12. P. 689436. doi: 10.3389/fimmu.2021.689436

[23] Shore PA, Burkhalter A, Cohn VHJr. A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther. 1959;127:182–186.

[24] Shore P.A., Burkhalter A., Cohn V.H.Jr. A method for the fluorometric assay of histamine in tissues // J Pharmacol Exp Ther. 1959. Vol. 127. P. 182–186.

[25] Galli SJ, Tsai M. IgE and mast cells in allergic disease. Nat Med. 2012;18(5):693–704. doi: 10.1038/nm.2755

[26] Galli S.J., Tsai M. IgE and mast cells in allergic disease // Nat Med. 2012. Vol. 18, N 5. P. 693–704. doi: 10.1038/nm.2755

[27] Fang Y, Larsson L, Bruhns P, Xiang Z. Apoptosis of mouse mast cells is reciprocally regulated by the IgG receptors FcγRIIB and FcγRIIIA. Allergy. 2012;67(10):1233–1240. doi: 10.1111/j.1398-9995.2012.02878.x

[28] Fang Y., Larsson L., Bruhns P., Xiang Z. Apoptosis of mouse mast cells is reciprocally regulated by the IgG receptors FcγRIIB and FcγRIIIA // Allergy. 2012. Vol. 67, N 10. P. 1233–1240. doi: 10.1111/j.1398-9995.2012.02878.x

[29] Jönsson F, Daëron M. Mast cells and company. Front Immunol. 2012;3:16. doi: 10.3389/fimmu.2012.00016

[30] Jönsson F., Daëron M. Mast cells and company // Front Immunol. 2012. Vol. 3. P. 16. doi: 10.3389/fimmu.2012.00016

[31] Junker F, Gordon J, Qureshi O. Fc gamma receptors and their role in antigen uptake, presentation, and T cell activation. Front Immunol. 2020;11:1393. doi: 10.3389/fimmu.2020.01393

[32] Junker F., Gordon J., Qureshi O. Fc gamma receptors and their role in antigen uptake, presentation, and T cell activation // Front Immunol. 2020. Vol. 11. P. 1393. doi: 10.3389/fimmu.2020.01393

RIGHTS & PERMISSIONS

Eco-Vector