Changes in progesterone and gonadoliberin levels after introduction of exogenous kisspeptin in Danio rerio females

Abstract

BACKGROUND: According to modern concepts, the kisspeptin signaling system is the upper (central) link in the regulation of reproductive function. The neuropeptide kisspeptin is considered an indicator of a number of obstetric pathologies in humans. Therefore, it should be considered important to search for new synthetic analogues of natural kisspeptins as pharmacological agents of reproduction regulation. On the other hand, it should also be considered important to search for new effective model organisms in which certain effects manifest themselves most prominently compared to traditional experimental animals.

AIM: To quantify the effect of kisspeptin-14 on the level of gonadoliberin and progesterone in two doses, at the time typical for the action of neuropeptides, on the model organism Danio rerio.

METHODS: In this study, an analogue of kisspeptin, conventionally designated kisspeptin-14, was used. All Danio rerio females had reached puberty by the time of the experiment (6–8 months). Kisspeptin was administered in doses of 1 and 4 ng. The groups were analyzed 1 and 4 hours after administration. Before administration of the preparations, the fish were subjected to lidocaine anesthesia at a concentration of 40 mg/l for 5–6 minutes, then the drug was administered intracerebroventrically. After waiting for 1 or 4 hours, the material was collected (gonads and midbrain). Gonad and midbrain samples were homogenized, homogenates were suspended, and enzyme immunoassay was performed to determine hormone concentrations.

RESULTS: Administration of kisspeptin-14 at a dose of 1 ng led to an increase in gonadoliberin levels 4 hours after administration. With a shorter time after administration, no statistically significant results were found. Administration of kisspeptin-14 at doses of 1 and 4 ng led to an increase in progesterone levels in the gonads of Danio rerio females 1 and 4 hours after administration. At the same time, statistically significant differences compared with the control group were noted at different sample collection dates.

CONCLUSION: Intracerebroventricular administration of the kisspeptin-14 at a dose of 1 ng after 4 hours causes an increase in gonadoliberin in the structures of the midbrain. Intracerebroventricular administration of kisspeptin-14 causes an increase in progesterone in the gonads of Danio rerio females. There was no unambiguous relationship between changes in the concentration of gonadoliberin in the structures of the midbrain and changes in the concentration of progesterone in the gonads of females.

Keywords

Danio rerio / kisspeptin / progesterone / gonadoliberin / zebrafish

References

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