Effect of intratumoral implantation of barium hexaferrite, magnetite, hematite, aluminium oxide and silica on the dynamics of Erlich tumor growth and survival value of tumor-carrying mice

Abstract

Introduction. We have earlier shown antitumoral effect of barium hexaferrite implant BHF for models of two intratumoral transplantable solid tumor of mice — LIO-1 and Erlich (2 sm³ tumors). In the mentioned work on the model of a rather big sized solid Erlich tumor (3 sm³) we have studied a comparative antitumoral effect after the implantation of BHF particles with a residual magnetic field of 150 mGsm referring to particles of iron oxides with residual magnetic field that was nearing to the zerohematite (Fe₂O₃) and magne — tite (Fe₃O₄), and as well as for the control — aluminium oxide (Al₂O₃) and silica (nSiO₂ ∙ mH₂O).

Material and methods. Trials have been conducted with 70 white mice — female mice with a mass of 23–24 g. Malignant epithelial tumor of Erlich was intramuscularly transplanted into the right hind extremity. When tumors reached a size of 3 sm³, mice were divided into 6 groups whom BHF, magnetite, hematite, aluminium oxide, silica or physiologic saline were implanted, correspondingly.

Results. As a result of the conducted studies it was found that BHF being implanted into a big-sized tumor (3 sm³) has a mild antitumoral effect for all the studies periods. Particles of iron oxides — magnetite and hematite — with a magnetic field nearing the zero did not possess the antitumor effect. By the end of experiment (29 days) in all groups including a control group, death of mice at 20 to 50 percent have been seen, and tumors were significantly necrotized, whereas in a group with implantation of silica into the tumor 100 percent of mice have survived and tumors itself did not have necrosis and had even pink colouring.

Keywords

Erlih tumor / implant / barium hexaferrite / magnetite / hematite / aluminium oxide / silica

References

[1] Заридзе Д.Г., Каприн А.Д., Стилиди И.С. Динамика заболеваемости злокачественными новообразованиями и смертности от них в России // Вопросы онкологии. – 2018. – Т. 64. – № 5. – С. 578–591. [Zaridze DG, Kaprin AD, Stilidi IS. Dynamics of morbidity and mortality from malignant tumors in Russia. Vopr Onkol. 2018;64(5):578-591. (In Russ.)]

[2] Каприн А.Д., Старинский В.В., Петрова Г.В. Злокачественные новообразования в России в 2016 году (заболеваемость и смертность). – М.: МНИОИ им. П.А. Герцена, 2018. – 250 с. [Kaprin AD, Starinskiy VV, Petrova GV. Zlokachestvennye novoobrazovaniya v Rossii v 2016 godu (zabolevaemost’ i smertnost’). Moscow: MNIOI im. P.A. Gertsena; 2018. 250 p. (In Russ.)]

[3] Гершанович М.Л., Филов В.А., Акимов М.А., Акимов А.А. Введение в фармакотерапию злокачественных опухолей. – СПб.: СОТИС, 1999. – 152 с. [Gershanovich ML, Filov VA, Akimov MA, Akimov AA. Vvedenie v farmakoterapiyu zlokachestvennykh opukholey. Saint Petersburg: SOTIS; 1999. 152 p. (In Russ.)]

[4] Евтушенко В.И., Вершинина С.Ф., Гранов А.М. Подавление роста и метастазирования злокачественных новообразований с помощью внутриопухолевой имплантации гексаферрита бария // Сибирский онкологический журнал. – 2009. – № S2. – С. 68. [Evtushenko VI, Vershinina SF, Granov AM. Podavlenie rosta i metastazirovaniya zlokachestvennykh novoobrazovaniy s pomoshch’yu vnutriopukholevoy implantatsii geksaferrita bariya. Sibirskii onkologicheskii zhurnal. 2009;(S2):68. (In Russ.)]

[5] Гранов А.М., Вершинина С.Ф., Маркочев А.Б., и др. Оценка противоопухолевого эффекта ферромагнитного имплантата в эксперименте // Медицинский академический журнал. – 2011. – Т. 11. – № 4. – С. 51–57. [Granov AM, Vershinina SF, Markochev AB, et al. Evaluation of antitumoral effect of ferromagnetic implant in the experiment. Med Akad Z. 2011;11(4):51-57. (In Russ.)]

[6] Гранов А.М., Карелин М.И., Гранов Д.А., и др. Магнитожесткий ферромагнетик в экспериментальной онкологии и клинике / под ред. А.М. Гранова. – СПб.: Фолиант, 2015. – 120 с. [Granov AM, Karelin MI, Granov DA, et al. Magnitozhestkiy ferromagnetik v eksperimental‘noy onkologii i klinike. Ed. by A.M. Granov. Saint Petersburg: Foliant; 2015. 120 p. (In Russ.)]

[7] Гранов А.М., Вершинина С.Ф., Самсонов Р.Б., и др. Противоопухолевый эффект имплантирования гексаферрита бария мышам SHR с односторонней и билатеральной лимфосаркомой ЛИО-1 // Медицинский академический журнал. – 2017. – Т. 17. – № 1. – С. 82–87. [Granov AM, Vershinina SF, Samsonov RB, et al. Antineoplastic effect of implantation of barium hexaferrite to shr mice suffering with unilateral and bilateral lymphosarcoma LIO-1. Med Akad Z. 2017;17(1):82-87. (In Russ.)]

[8] Kim JS, Yoon TJ, Yu KN, et al. Toxicity and tissue distribution of magnetic nanoparticles in mice. Toxicol Sci. 2006;89(1):338-347. https://doi.org/10.1093/toxsci/kfj027.

[9] Вершинина С.Ф., Стуков А.Н. Экспериментальные опухоли: практическое руководство. – СПб.: Галаника, 2018. – 68 с. [Vershinina SF, Stukov AN. Eksperimental’nye opukholi. Prakticheskoe rukovodstvo. Saint Petersburg: Galanika; 2018. 68 p. (In Russ.)]

[10] Стуков А.Н., Иванова М.А., Никитин А.К., и др. Индекс роста опухоли как интегральный критерий эффективности противоопухолевой терапии в эксперименте // Вопросы онкологии. – 2001. – Т. 47. – № 5. – С. 616–618. [Stukov AN, Ivanova MA, Nikitin AK, et al. Indeks rosta opukholi kak integral’nyy kriteriy effektivnosti protivoopukholevoy terapii v eksperimente. Vopr Onkol. 2001;47(5):616-618. (In Russ.)]

RIGHTS & PERMISSIONS

Vershinina S.F., Evtushenko V.I.