THE OXIDATIVE STATUS OF BLOOD AND TISSUES IN INFLAMMATORY AND DEGENERATIVE DAMAGE TO THE SMOOTH PARODENTIUM TISSUE IN RATS AFTER USING OF TOOTH PASTES AND THEIR SEPARATE COMPONENTS

Abstract

A rat model of inflammatory and degenerative damage to the smooth parodentium tissue, which is produced by administering 2% aqueous formaldehyde into the smooth parodentium, is described. The oxidative status of rats, including lipid peroxidation and antioxidant system parameters, was assessed because of its high sensitivity to inflammation. Malondialdehyde and diene conjugates levels were 2- to 3-fold higher in blood serum and parodentium tissue 7 days after the onset of inflammation. Reduced glutathion level and superoxide dismutase activity were somewhat more sensitive than the parameters of lipid peroxidation: blood serum reduced glutathione decreased from 2,02±0,09 to 0,88±0,21 pM/mL, and superoxide dismutase activity decreased from 0,78±0,10 to 0,28±0,04 unit/mg protein, that is, decreased 2,5 to 3 times. Similar changes were observed in the smooth parodentium: reduced glutathione decreased 2,1-fold, and superoxide dismutase activity decreased 7-fold. The toothpastes Lesmin-Dent,Clamin-Dent and Zhemchug (in the order of descending activity) attenuated or abolished the signs of inflammatory and degenerative damages to the smoothparodentium tissue in rats, as assessed by lipid peroxidation and antioxidant defense parameters. The antihypoxic drugs Midalon, Elam-A, and Elam-M (in the order of descending activity) after 7 days of oral administration attenuated the signs ofinflammatory and degenerative damage to the smooth parodentium more than the toothpastes did. Therefore, the curative and preventive effects of toothpastes and dental drugs may be assessed using the above model of inflammation.

Keywords

parodentium / inflammation / oxidative stress / lipid peroxidation / antioxidant systems / tooth pastes / antihypoxants / Midalon

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RIGHTS & PERMISSIONS

Shabanov P.D., Mokrenko Y.V.