Diurnal dynamics of rat blood electrolytes at constant lighting and chronic alcohol intoxication

Abstract

BACKGROUND: Violation of the lighting regime, as well as alcohol abuse, is significant factors in the disorganization of biorhythms in the modern world. Violation of rhythm leads to a mismatch of body functions, as a result to disadaptation, the occurrence of desynchronosis and the development of a number of socially significant diseases.

AIM: Study of influence of constant lighting, chronic alcohol intoxication and joint action of these factors on diurnal dynamics of several electrolytes in blood of rats.

MATERIALS AND METHODS: The study was conducted on 160 male of Wistar outbred stock at age of 6 months, with body weight of 300 ± 20 g, which were divided into 4 groups. Control group — animals, which were kept under conditions of fixed light regime (light:darkness = 10:14 hours), I group — animals, which were kept under conditions of fixed light regime, but received a 15% water solution of ethanol as a drink, II group — animals, which were kept under conditions of constant light regime, III group — animals, which were kept under conditions of constant light regime and received a 15% water solution of ethanol as a drink. In blood samples, taken at 9:00, 15:00, 21:00 and 3:00 o’clock, the concentration of total calcium, potassium, sodium, chlorine and phosphorus was measured. The reliability of circadian rhythmicity was determined through cosinor analysis.

RESULTS: The increase in the concentration of phosphorus in the blood plasma of animals of II and III experimental groups was found with the decrease in the concentration of total calcium in animals of I and III experimental groups. Constant lighting and chronic alcohol intoxication affect the amplitude-phase characteristics of the circadian rhythms of all studied ions, and the combined action of these factors causes the destruction of the circadian rhythms of the studied electrolytes.

CONCLUSIONS: It is established that the three-weeks-long influence of the studied chronodestructors leads to disruptions in metabolism of calcium and phosphorus, which are the most pronounced at joint action of darkness deprivation and chronic alcohol intoxication, and also to the significant disruptions in the structure of CRs of all of studied substances.

Keywords

chronic alcohol intoxication / calcium / sodium / potassium / chlorine / phosphorus / circadian rhythm / cosinor

References

[1] Dibner C, Schibler U, Albrecht U. The mammalian circadian timing system: organization and coordination of central and peripheral clocks. Annu Rev Physiol. 2010;72:517–549. DOI: 10.1146/annurev-physiol-021909-135821

[2] Dibner C., Schibler U., Albrecht U. The mammalian circadian timing system: organization and coordination of central and peripheral clocks // Annu. Rev. Physiol. 2010. Vol. 72. P. 517–549. DOI: 10.1146/annurev-physiol-021909-135821

[3] Finger AM, Dibner C, Kramer A. Coupled network of the circadian clocks: a driving force of rhythmic physiology. FEBS letters. 2020;594(17):2734–2769. DOI: 10.1002/1873-3468.13898

[4] Finger A.M., Dibner C., Kramer A. Coupled network of the circadian clocks: a driving force of rhythmic physiology // FEBS letters. 2020. Vol. 594, No. 17. P. 2734–2769. DOI: 10.1002/1873-3468.13898

[5] Green CB. Circadian posttranscriptional regulatory mechanisms in mammals. Cold Spring Harbor Perspect Biol. 2018;10(6):a030692. DOI: 10.1101/cshperspect.a030692

[6] Green C.B. Circadian posttranscriptional regulatory mechanisms in mammals // Cold Spring Harbor Perspect. Biol. 2018. Vol. 10, No. 6. P. a030692. DOI: 10.1101/cshperspect.a030692

[7] Thurley K, Herbst C, Wesener F, et al. Principles for circadian orchestration of metabolic pathways. Proc Natl Acad Sci USA. 2017;114(7):1572–1577. DOI: 10.1073/pnas.1613103114

[8] Thurley K., Herbst C., Wesener F. et al. Principles for circadian orchestration of metabolic pathways // Proc. Natl. Acad. Sci. USA. 2017. Vol. 114, No. 7. P. 1572–1577. DOI: 10.1073/pnas.1613103114

[9] Jacobsen AA, Bressendorff I, Nordholm A, et al. Diurnal variation of magnesium and the mineral metabolism in patients with chronic kidney disease. Bone Rep. 2021;15:101130. DOI: 10.1016/j.bonr.2021.101130

[10] Jacobsen A.A., Bressendorff I., Nordholm A. et al. Diurnal variation of magnesium and the mineral metabolism in patients with chronic kidney disease // Bone Rep. 2021. Vol. 15. P. 101130. DOI: 10.1016/j.bonr.2021.101130

[11] Palmer BF, Clegg DJ. Electrolyte disturbances in chronic alcohol-use disorder. N Engl J Med. 2018;378(2):203–204. DOI: 10.1056/NEJMc1714331

[12] Palmer B.F., Clegg D.J. Electrolyte disturbances in chronic alcohol-use disorder // N. Engl. J. Med. 2018. Vol. 378, No. 2. P. 203–204. DOI: 10.1056/NEJMc1714331

[13] Baj J, Flieger W, Teresiński G, et al. Magnesium, calcium, potassium, sodium, phosphorus, selenium, zinc, and chromium levels in alcohol use disorder: a review. J Clin Med. 2020;9(6):1901. DOI: 10.3390/jcm9061901

[14] Baj J., Flieger W., Teresiński G. et al. Magnesium, calcium, potassium, sodium, phosphorus, selenium, zinc, and chromium levels in alcohol use disorder: a review // J. Clin. Med. 2020. Vol. 9, No. 6. P. 1901. DOI: 10.3390/jcm9061901

[15] Fárková E, Schneider J, Šmotek M, et al. Weight loss in conservative treatment of obesity in women is associated with physical activity and circadian phenotype: a longitudinal observational study. Biopsychosoc Med. 2019;13:24. DOI: 10.1186/s13030-019-0163-2

[16] Fárková E., Schneider J., Šmotek M. et al. Weight loss in conservative treatment of obesity in women is associated with physical activity and circadian phenotype: a longitudinal observational study // Biopsychosoc. Med. 2019. Vol. 13. P. 24. DOI: 10.1186/s13030-019-0163-2

[17] Davis BT 4th, Voigt RM, Shaikh M, et al. Circadian mechanisms in alcohol use disorder and tissue injury. Alcohol Clin Exp Res. 2018;42(4):668–677. DOI: 10.1111/acer.13612

[18] Davis B.T. 4th, Voigt R.M., Shaikh M. et al. Circadian mechanisms in alcohol use disorder and tissue injury // Alcohol. Clin. Exp. Res. 2018. Vol. 42, No. 4. P. 668–677. DOI: 10.1111/acer.13612

[19] Cornelissen G. Cosinor-based rhythmometry. Theor Biol Med Model. 2014;11:16. DOI: 10.1186/1742-4682-11-16

[20] Cornelissen G. Cosinor-based rhythmometry // Theor. Biol. Med. Model. 2014. Vol. 11. P. 16. DOI: 10.1186/1742-4682-11-16

[21] Peng TAIC, Gitelman H. Ethanol-induced hypocalcemia, hypermagnesemia and inhibition of the serum calcium-raising effect of parathyroid hormone in rats. Endocrinology. 1974;94(2):608–611. DOI: 10.1210/endo-94-2-608

[22] Peng T.A.I.C., Gitelman H. Ethanol-induced hypocalcemia, hypermagnesemia and inhibition of the serum calcium-raising effect of parathyroid hormone in rats // Endocrinology. 1974. Vol. 94, No. 2. P. 608–611. DOI: 10.1210/endo-94-2-608

[23] Beto JA. The role of calcium in human aging. Clin Nutr Res. 2015;4(1):1–8. DOI: 10.7762/cnr.2015.4.1.1

[24] Beto J.A. The role of calcium in human aging // Clin. Nutr. Res. 2015. Vol. 4, No. 1. P. 1–8. DOI: 10.7762/cnr.2015.4.1.1

[25] Chu ZM, Li HB, Sun SX, et al. Melatonin promotes osteoblast differentiation of bone marrow mesenchymal stem cells in aged rats. Eur Rev Med Pharmacol Sci. 2017;21(19):4446–4456.

[26] Chu Z.M., Li H.B., Sun S.X. et al. Melatonin promotes osteoblast differentiation of bone marrow mesenchymal stem cells in aged rats // Eur. Rev. Med. Pharmacol. Sci. 2017. Vol. 21, No. 19. P. 4446–4456.

[27] Shinoda H, Seto H. Diurnal rhythms in calcium and phosphate metabolism in rodents and their relations to lighting and feeding schedules. Miner Electrolyte Metab. 1985;11(3):158–166.

[28] Shinoda H., Seto H. Diurnal rhythms in calcium and phosphate metabolism in rodents and their relations to lighting and feeding schedules // Miner. Electrolyte Metab. 1985. Vol. 11, No. 3. P. 158–166.

[29] Harvey JR, Plante AE, Meredith AL. Ion channels controlling circadian rhythms in suprachiasmatic nucleus excitability. Physiol Rev. 2020;100(4):1415–1454. DOI: 10.1152/physrev.00027.2019

[30] Harvey J.R., Plante A.E., Meredith A.L. Ion channels controlling circadian rhythms in suprachiasmatic nucleus excitability // Physiol. Rev. 2020. Vol. 100, No. 4. P. 1415–1454. DOI: 10.1152/physrev.00027.2019

[31] Beck L, Beck-Cormier S. Extracellular phosphate sensing in mammals: what do we know? J Mol Endocrinol. 2020;65(3):R53–R63. DOI: 10.1530/JME-20-0121

[32] Beck L., Beck-Cormier S. Extracellular phosphate sensing in mammals: what do we know? // J. Mol. Endocrinol. 2020. Vol. 65, No. 3. P. R53–R63. DOI: 10.1530/JME-20-0121

[33] Portale AA, Halloran BP, Morris RC. Dietary intake of phosphorus modulates the circadian rhythm in serum concentration of phosphorus. Implications for the renal production of 1,25-dihydroxyvitamin D. J Clin Invest. 1987;80(4):1147–1154. DOI: 10.1172/jci113172

[34] Portale A.A., Halloran B.P., Morris R.C. Dietary intake of phosphorus modulates the circadian rhythm in serum concentration of phosphorus. Implications for the renal production of 1,25-dihydroxyvitamin D // J. Clin. Invest. 1987. Vol. 80, No. 4. P. 1147–1154. DOI: 10.1172/jci113172

[35] Roman E, Karlsson O. Increased anxiety-like behavior but no cognitive impairments in adult rats exposed to constant light conditions during perinatal development. Ups J Med Sci. 2013;118(4):222–227. DOI: 10.3109/03009734.2013.821191

[36] Roman E., Karlsson O. Increased anxiety-like behavior but no cognitive impairments in adult rats exposed to constant light conditions during perinatal development // Ups. J. Med. Sci. 2013. Vol. 118, No. 4. P. 222–227. DOI: 10.3109/03009734.2013.821191

[37] Gumz ML, Rabinowitz L. Role of circadian rhythms in potassium homeostasis. Semin Nephrol. 2013;33(3):229–236. DOI: 10.1016/j.semnephrol.2013.04.003

[38] Gumz M.L., Rabinowitz L. Role of circadian rhythms in potassium homeostasis // Semin. Nephrol. 2013. Vol. 33, No. 3. P. 229–236. DOI: 10.1016/j.semnephrol.2013.04.003

[39] Guo H, Brewer JM, Champhekar A, et al. Differential control of peripheral circadian rhythms by suprachiasmatic-dependent neural signals. Proc Natl Acad Sci USA. 2005;102(8):3111–3116. DOI: 10.1073/pnas.0409734102

[40] Guo H., Brewer J.M., Champhekar A. et al. Differential control of peripheral circadian rhythms by suprachiasmatic-dependent neural signals // Proc. Natl. Acad. Sci. USA. 2005. Vol. 102, No. 8. P. 3111–3116. DOI: 10.1073/pnas.0409734102

[41] Poulis JA, Roelfsema F, van Der Heide D. Circadian urinary excretion rhythms in adrenalectomized rats. Am J Physiol. 1986;251(3 Pt 2):R441–R449. DOI: 10.1152/ajpregu.1986.251.3.R441

[42] Poulis J.A., Roelfsema F., van Der Heide D. Circadian urinary excretion rhythms in adrenalectomized rats // Am. J. Physiol. 1986. Vol. 251, No. 3 Pt 2. P. R441–R449. DOI: 10.1152/ajpregu.1986.251.3.R441

[43] Nikolaeva S, Pradervand S, Centeno G, et al. The circadian clock modulates renal sodium handling. J Am Soc Nephrol. 2012;23(6):1019–1026. DOI: 10.1681/ASN.2011080842

[44] Nikolaeva S., Pradervand S., Centeno G. et al. The circadian clock modulates renal sodium handling // J. Am. Soc. Nephrol. 2012. Vol. 23, No. 6. P. 1019–1026. DOI: 10.1681/ASN.2011080842

[45] Tsuda T, Ide M, Iigo M. Influences of season and of temperature, photoperiod, and subcutaneous melatonin infusion on the glomerular filtration rate of ewes. J Pineal Res. 1995;19(4):166–172. DOI: 10.1111/j.1600-079X.1995.tb00185.x

[46] Tsuda T., Ide M., Iigo M. Influences of season and of temperature, photoperiod, and subcutaneous melatonin infusion on the glomerular filtration rate of ewes // J. Pineal Res. 1995. Vol. 19, No. 4. P. 166–172. DOI: 10.1111/j.1600-079X.1995.tb00185.x

[47] Kozlova MА, Kirillov YА, Makartseva LА, et al. Morphofunctional state and circadian rhythms of the liver under the influence of chronic alcohol intoxication and constant lighting. Int J Mol Sci. 2021;22(23):13007. DOI: 10.3390/ijms222313007

[48] Kozlova M.А., Kirillov Y.А., Makartseva L.А. et al. Morphofunctional state and circadian rhythms of the liver under the influence of chronic alcohol intoxication and constant lighting // Int. J. Mol. Sci. 2021. Vol. 22, No. 23. P. 13007. DOI: 10.3390/ijms222313007

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Kirillov Y.A., Makartseva L.A., Kozlova M.A., Muratova M.V., Chernov I.A., Areshidze D.A.