Effect of experimental therapy on the gas and acid–base composition of the blood in the dynamics of toxic pulmonary edema

Abstract

BACKGROUND: This study is part of an extended research onfinding ways to prevent and treat toxic pulmonary edema. This study aimed to examine the gas transport function, acid–base state, and gas composition of blood in the dynamics of experimental toxic pulmonary edema and the effect on them of experimental therapy with a combination of drugs that have shown effectiveness in preliminary studies.

MATERIALS AND METHODS: Toxic edema of the lungs was modeled in mice by inhalation of toxic doses of phosgene LСt50. Gas and acid–base composition parameters of the blood were determined using a gas analyzer 3 and 24 h after poisoning. Thirty minutes after poisoning, parts of the animals were injected intraperitoneally with a complex of drugs consisting of natrii dimercaptopropansulfonas (unitiol) 150 mg/kg, diclofenac sodium 35. 0 mg/kg, and аprotinin (contrikal) 250 IU/kg.

RESULTS: The results revealed 3 h after poisoning of animals, changes in the gas composition and parameters of the gas transport function in the blood were detected, and 24 h after poisoning, impaired acid-base balance was noted. The use of a therapeutic combination of drugs consisting of sodium dimercaptopropanesulfonate (unithiol), sodium diclofenac, and aprotinin (kontrikal) led to the partial normalization of the altered parameters of the blood gas composition and parameters of the blood gas transport function but was not accompanied by the normalization of the acid-base state of the blood. The formulation demonstrated a pronounced negative effect on the blood gas composition in intact animals. The treatment of pulmonary edema with the comparison drug unithiol did not result in the normalization of the gas composition and acid-base state of the poisoned animals.

CONCLUSION:The treatment of toxic pulmonary edema with a combination of medications led to an improvement in the gas composition and gas transport function of the blood.

Keywords

pulmonary edema / phosgene / poisoning / blood gases / acid-base composition of blood / experimental treatment / unithiol / diclofenac sodium / aprotinin

References

[1] Ryabov GA. Sindromy kriticheskikh sostoyanii. Moscow: Meditsina; 1994. 368 p. (In Russ. )

[2] Рябов Г. А. Синдромы критических состояний. Москва: Медицина, 1994. 368 с.

[3] Tomchin AB, Kropotov AV. Proizvodnye tiomocheviny i tiosemikarbazida. Stroenie i farmakologicheskaya aktivnost’. Zashchitnoe deistvie proizvodnykh 1,2,4-tiazinoindola pri oteke legkikh. Khimiko-Farmatsevticheskii Zhurnal. 1998;(1):22–26. (In Russ. )

[4] Томчин А. Б., Кропотов А. В. Производные тиомочевины и тиосемикарбазида. Строение и фармакологическая активность. Защитное действие производных 1,2,4-тиазиноиндола при отеке легких // Химико-фармацевтический журнал. 1998. № 1. С. 22–26.

[5] Kropotov AV. Ehksperimental’nyi otek legkikh i ego farmakoprofilaktika antigipoksantami [dissertation Abstract]. Saint Petersburg; 1996. 44 p. (In Russ. )

[6] Кропотов А. В. Экспериментальный отек легких и его фармакопрофилактика антигипоксантами: автореф. дис. … д-ра мед. наук. Санкт-Петербург, 1996. 44 с.

[7] Kaare E. Lundstrom. The blood gas handbook. Bronshoj; 1997. 58 p.

[8] Kaare E. Lundstrom. The blood gas handbook. Bronshoj, 1997. 58 p.

[9] Komarov FI, Korovkin BF, Men’shikov VV. Biokhimicheskie issledovaniya v klinike. Leningrad: Meditsina; 1981. 408 p. (In Russ. )

[10] Комаров Ф. И., Коровкин Б. Ф., Меньшиков В. В. Биохимические исследования в клинике. Ленинград: Медицина, 1981. 408 с.

[11] Shanin VYu. Klinicheskaya patofiziologiya. Uchebnik dlya meditsinskikh vuzov. Saint Petersburg: Spetsial’naya literatura; 1998. 569 p. (In Russ. )

[12] Шанин В. Ю. Клиническая патофизиология. Учебник для медицинских вузов. Санкт-Петербург: Специальная Литература, 1998. 569 с.

[13] Motavkin PA, Gel’tser BI. Klinicheskaya i ehksperimental’naya patofiziologiya legkikh. Moscow: Nauka; 1998. 366 p. (In Russ. )

[14] Мотавкин П. А., Гельцер Б. И. Клиническая и экспериментальная патофизиология легких. Москва: Наука, 1998. 366 с.

[15] Torkunov PA, Zemlyanoy AV, Varlashova MB, et al. Experimental therapy for toxic pulmonary edema caused by inhalation poisoning with nitrogen oxides. Psychopharmacology and biological narcology. 2023;14(1):62–69. (In Russ. ) DOI: 10. 17816/phbn321622

[16] Торкунов П. А., Земляной А. В., Варлашова М. Б., и др. Экспериментальная терапия токсического отека легких, вызванного ингаляционным отравлением оксидами азота // Психофармакология и биологическая наркология. 2023. Т. 14, № 1. С. 62–69. DOI: 10. 17816/phbn321622

[17] Topunov AF, Kosmachevskaya OV. The multiplicity of hemoglobin functional forms in human organism: the modern view and practical applications. Biomics. 2018;10(3):251–267. (In Russ. ) DOI: 10. 31301/2221-6197. bmcs. 2018-34

[18] Топунов А. Ф., Космачевская О. В. Множественные функциональные формы гемоглобина в организме человека: современный взгляд и практическое использование // Биомика. 2018. Т. 10, № 3. С. 251–267. DOI: 10. 31301/2221-6197. bmcs. 2018-34

[19] Suslikova MI, Gubina MI, Aleksandrov SG, et al. Izbrannye voprosy fiziologii krovi. Uchebnoe posobie. Irkutsk: IGMU; 2021. 102 p. (In Russ. )

[20] Сусликова М. И., Губина М. И., Александров С. Г., и др. Избранные вопросы физиологии крови. Учебное пособие. Иркутск: ИГМУ, 2021. 102 с.

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