Experimental therapy for toxic pulmonary edema caused by inhalation poisoning with nitrogen oxides

Abstract

BACKGROUND: Morphological studies conducted in different phases of toxic pulmonary edema initiated by nitrogen oxides have revealed changes in blood vessels and blood cells that develop at the earliest stages of the lesion and precede edema.

AIM: To examine the cellular composition of the blood of mice in the presence of inhalation poisoning with nitrogen oxides.

MATERIALS AND METHODS: The toxic lung edema was modeled in mice by inhalation of toxic doses of nitrogen oxides LСt₅₀. Blood cells were determined using a hematological analyzer 0.5, 3, and 24 h after poisoning. Parts of the animals, 30 min after the poisoning, were injected intraperitoneally with a complex of drugs consisting of sodium dimercaptopropane sulfonate (unithiol) 150 mg/kg, diclofenac sodium 35.0 mg/kg, and аprotinin (contrikal) 250 IU / kg.

RESULTS: In mice poisoned with nitrogen oxides, the main differences were observed in the leukocyte count, composition of the leukocyte formula, and platelet count. Thrombocytosis is observed 3 and 24 h after intoxication. The earliest manifestation of blood poisoning (0.5 h) was a decrease in the total leukocyte count (leukopenia). During the pronounced clinical manifestations of nitric oxide poisoning (3 h), a change in the leukocyte formula toward an increase in the proportion of granulocytes and “medium” cells (granulocytosis and eosinophilia-monocytosis) was noted. A day after the poisoning, the noted indicators generally return to the initial level. Treatment of poisoned animals with a combination of drugs neutralizes the observed effects.

CONCLUSION: The combination of drugs consisting of sodium dimercaptopropane sulfonate (unithiol), diclofenac sodium, and аprotinin (contrikal) was found to be effective in the treatment of toxic lung edema induced by nitrogen oxides.

Keywords

nitric oxides / poisoning / blood cells / experimental treatment / unithiol / diclofenac sodium / contrikal

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