Expression of heat shock proteins HSP90 on neutrophils peripheral blood in patients with atopic dermatitis

Abstract

HSP90 expression on the neutrophil plasma membrane has been investigated in patients with atopic dermatitis. Methods. Human peripheral blood cells were stained with monoclonal antibodies to the HSP90 and were analyzed using the flow cytometer. Results. Neutrophils obtained from patients with atopic dermatitis have been found to express the more significant level of HSP90 on plasma membrane, and percentage of these cells has been shown to be higherfor atopic patients compared with healthy donors. Both, neutrophil count and HSP90 fluorescence were increased according elevation of index SCORAD for atopic patients. The most significant elevation in percentage of neutrophils expressing HSP90 on plasma membrane has been found during the first and second weeks of acute phase of the disease. We did not found the significant differences for HSP90 expression on neutrophils dependent on IgE level in the blood serum. Conclusion. HSP90 expression is elevated on the neutrophil plasma membrane from atopic dermatitis patients compared with healthy donors. Percentage of these cells were positive correlated with SCORAD index.

Keywords

atopic dermatitis / heat shock protein 90 (HSP90) / neutrophils

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