INTESTINAL MICROBIOCENOSIS AND CYTOKINE STATUS IN LUNG CANCER PATIENTS RECEIVING CHEMOTHERAPY

Abstract

In patients with oncological diseases chemotherapy leads to the damage of a mucous membrane of gastrointestinal tract, as well as to a deterioration of the intestinal microbiocenosis. The article presents the changes in intestinal microbiocenosis in lung cancer patients, chemotherapy, the nature of the influence appointed in the schemes of the treatment of lung cancer, impact of anticancer drugs on the state of the intestinal microflora, and the improvement of technologies of treatment of lung cancer patients receiving chemotherapy on the basis of supplementation of the complex therapy by the probiotics Material and methods. 41 lung cancer patient receiving the first line of the first cycle of chemotherapy was included. The age of patients varied from 49 to 73 years, the average duration of the disease was 1 year. Patients from the main group (n = 21) received probiotics treatment together with the chemotherapy course. Patients from the control group (n = 20) received only chemotherapeutic preparations. All patients were observed before and after treatment, the study of metabolites of intestinal microorganisms in blood was performed by the method of the gas-liquid chromatography - mass-spectrometry by G.A. Osipov’s method, determination of cytokine status multiplex method. The efficiency of probiotic therapy was evaluated by results of the dynamics of studied indices. Results. The deterioration in intestinal microflora was manifested as the decreased quantity of Lactobacillus, Bifidobacterium, and increased quantity of different pathogenic microorganisms. It was noted decreased rate in the improvement of composition intestinal microflora after the treatment course with the metabiotic. Conclusion. Using of metabiotic medicines with the chemotherapy in lung cancer patients is promising to prevent deterioration of the gut microflora.

Keywords

lung cancer / chemotherapy / intestinal dysbiosis / intestinal microbiota / gas-liquid chromatography - mass spectrometry of microbe markers / determination of cytokine status multiplex method / metabiotic

References

[1] Carding S., Verbeke K., Vipond D.T., Corfe B.M., Owen L.J. Dysbiosis of the gut microbiota in disease. Microb. Ecol. Health Dis. 2015 - online Feb 2. doi: 10.3402/mehd.v26.26191.

[2] Hooper L.V., Macpherson A.J. Immune adaptations that maintain homeostasis with the intestinal microbiota. Nat. Rev. Immunol. 2010; 10: 159-69.

[3] Stokes C.R. The development and role of microbial-host interactions in gut mucosal immunedevelopment. J. Anim. Sci. Biotechnol. 2017. - online published Jan 26; 8: 12, doi: 10.1186/s40104-016-0138-0. eCollection 2017.

[4] Серкова М.Ю., Уртенова М.А., Ткаченко Е.И., Авалуева Е.Б., Орлов С.В., Иванов С.В. и др. К вопросу о возможностях коррекции изменений микробиоты гастроинтестинального тракта у пациентов с раком лёгкого, получающих химиотерапию. Экспериментальная и клиническая гастроэнтерология. 2013; (11): 15-20.

[5] Серкова М.Ю., Ткаченко Е.И., Авалуева Е.Б., Орлов С.В., Иванов С.В., Шевяков М.А. Кишечный биотоп у пациентов с раком лёгкого, получающих химиотерапию. Проблемы медицинской микологии. 2014; 16 (4): 8-12.

[6] Abrea M.T., Fukata M, Arditi M. TLR signaling in the gut in health and disease. J. Immunol. 2005; 174 (8): 4453-60.

[7] Бакулин И.Г., Шкурко Т.В., Парфенов А.И., Князев О.В., Фадеева Н.А., Жулина Е.Ю. и др. К вопросу о распространённости и заболеваемости воспалительных заболеваний кишечника в Москве. Фарматека. Гастроэнтерология/Гепатология. 2016; (2): 69-73.

[8] Kamada N., Núñez G. Role of the Gut Microbiota in the Development and Function of Lymphoid Cells. J. Immunol. 2013; 190 (4): 1389-95. doi: 10.4049/jimmunol.1203100.

[9] Шитов Л. Н., Романов В.А. Влияние иммунодепрессантов на популяционный уровень и адгезивные свойства условно-патогенных бактерий толстой кишки белых мышей. Журн. микробиологии, эпидемиологии и иммунобиологии. 2009; (6): 12-6.

[10] Ефремова Н.В., Солдатова Г.С., Виноградов С.П., Бурундукова М.В. Клиническая и микробиологическая характеристики поражения толстой кишки у больных лимфомами в ранний и отдалённый периоды клинико-гематологической ремиссии. Бюллетень СО РАМН. 2011; 31 (2): 41-7.

[11] Bosscher D. et al. Microbiota and colonic cancer effects. J. of Physiology and Pharmacology. 2009; 60 (Suppl. 6): 9-13.

[12] van Vliet M.J., Harmsen H.J., de Bont E.S., Tissing W.J. The Role of Intestinal Microbiota in the Development and Severity of Chemotherapy-Induced Mucositis. J. PLoS Pathog. 2010; 6: 1045-60.

[13] Корниенко Е.А., Дроздова С.Н., Серебряная Н.Б. Пробиотики как способ повышения эффективности эрадикации Helicobacter pylori у детей. Фарматека. 2005; 7: 68-70.

[14] Yan F., Polk D.B. Probiotic bacterium prevenrs cytokine-induced apoptosis in intestinal epithelial cells. J. Biol. Chem. 2002; 277: 50 959-65.

[15] Kinross J., von Roon A.C., Penney N., Holmes E., Silk D., Nicholson J.K., Darzi A. The gut microbiota as a target for improved surgical outcome and improved patient care. Curr Pharm Des. 2009; 15 (13): 1537-45.

[16] Осипов Г.А. Хромато-масс-спектрометрический анализ микроорганизмов и их сообществ в клинических пробах при инфекциях и дисбиозах. В кн.: Химический анализ в медицинской диагностике. М.: Наука; 2010: 293-368.

[17] Гржибовский А.М. Типы данных, проверка распределения и описательная статистика. Экология человека. 2008; (1): 52-8.

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