APPLICATION OF ELISA-IGM (MAC-ELISA) FOR DETERMINING ETIOLOGICAL LINK VIRUS TYPES IN CONCRET CASES OF THE DISEASE

Yuliya A. Akinshina , V. F Larichev , M. A Sayfullin , S. G Mardanly , A. M Butenko

Epidemiology and Infectious Diseases ›› 2017, Vol. 22 ›› Issue (3) : 116 -121.

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Epidemiology and Infectious Diseases ›› 2017, Vol. 22 ›› Issue (3) : 116 -121. DOI: 10.17816/EID40955
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APPLICATION OF ELISA-IGM (MAC-ELISA) FOR DETERMINING ETIOLOGICAL LINK VIRUS TYPES IN CONCRET CASES OF THE DISEASE

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Abstract

105 sera from 101 patients with a confirmed diagnosis of dengue fever (LD) were examined using monospecific ELISA-IgM test. Monospecific results were observed in 39 samples (37.1%). 27 sample of them were taken during the first 7 days of the disease. In those systems were examined 23 serum samples of 23 patients, in which etiological role of one of the four dengue viruses was been established by the RT-PCR method. In this case, the coincidence-IgM ELISA results and the RT-PCR took place in 14 sera (60.9%).

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dengue fever / ELISA-IgM / RT-PCR / differential serological diagnosis / dengue viruses

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Yuliya A. Akinshina, V. F Larichev, M. A Sayfullin, S. G Mardanly, A. M Butenko. APPLICATION OF ELISA-IGM (MAC-ELISA) FOR DETERMINING ETIOLOGICAL LINK VIRUS TYPES IN CONCRET CASES OF THE DISEASE. Epidemiology and Infectious Diseases, 2017, 22(3): 116-121 DOI:10.17816/EID40955

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References

[1]

ВОЗ. Денге и тяжелая денге. Информационный бюллетень. 2015; (117).

[2]

Weiskopf D., Angelo M.A., Sidney J., Peters B., Shresta S., Sette A. Immunodominance changes as a function of the infecting dengue virus serotype and primary versus secondary infection. J. Virol. 2014; 88 (19): 11 383-94.

[3]

Ларичев В.Ф., Сайфуллин М.А., Акиншина Ю.А., Хуторецкая Н.В., Бутенко А.М. Завозные случаи арбовирусных инфекций в Российской Федерации. Эпидемиол. и инфекц. бол. 2012; (1): 35-9.

[4]

Akehurst C. Dengue virus genotypes and haemorrhagic fever. Eurosurveillance. 1999; 3 (44): pii=1309.

[5]

Kochel T.J., Watts D.M., Gozalo A.S., Ewing D.F., Porter K.R., Russell K.L. Cross-serotype neutralization of dengue virus in aotus nancymae monkeys. J. Infect. Dis. 2005; 191 (6): 1000-4.

[6]

Vorndam V., Kuno G., Gubler D.J., Kuno G. Dengue and Dengue Hemorrhagic Fever. New York: CAB International. Laboratory Diagnosis of Dengue Virus Infections; 1997: 313-33.

[7]

Najioullah F., Viron F., Césaire R. Evaluation of four commercial real-time RT-PCR kits for the detection of dengue viruses in clinical samples. Virol. J. 2014; 11 (1): 164.

[8]

WHO. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. New Edition. 2009. ISBN 978-9-241547-87-1

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