COMPARISON OF THE APPLICATION OF DOMESTIC «ELISA-IGM-DENGUE» KIT, DELIVERED IN THE D.I. IVANOVSKY INSTITUTE OF VIROLOGY (MOSCOW, RUSSIAN FEDERATION) AND «ANTI-DENGUE VIRUS ELISA IGM» KIT (EUROIMMUN, GERMANY) FOR THE SERODIAGNOSIS OF DENGUE FEVER

Yu. A Akinshina; V. F Larichev; M. A Saifullin; S. G Mardanly; A. M Butenko

doi:10.17816/EID40949

Epidemiology and Infectious Diseases ›› 2017, Vol. 22 ›› Issue (1) : 4 -8. DOI: 10.17816/EID40949

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COMPARISON OF THE APPLICATION OF DOMESTIC «ELISA-IGM-DENGUE» KIT, DELIVERED IN THE D.I. IVANOVSKY INSTITUTE OF VIROLOGY (MOSCOW, RUSSIAN FEDERATION) AND «ANTI-DENGUE VIRUS ELISA IGM» KIT (EUROIMMUN, GERMANY) FOR THE SERODIAGNOSIS OF DENGUE FEVER

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88 sera from patients with dengue fever were examined with the use of two test systems: Pilot Production kit «ELISA- IgM-dengue», delivered in the D.I. Ivanovsky Institute of Virology (Russian Federation) and. All 88 samples were positive for specific IgM in «ELISA-IgM-dengue», but 8 cases examined with the test kit «Anti-Dengue virus ELISA (IgM)» (Euroimmun, Germany) appeared to be negative. The ratio between the titers of anti-dengue IgM determined in patients’ blood samples by "ELISA-IgM-dengue" and «Ratio» (index, recommended by the company «Euroimmun» for differentiation of positive, equivocal or negative results, was evaluated. 53 blood sample containing M antibodies to cytomegalovirus (n = 43), Epstein-Barr virus (n = 6), herpes simplex virus (n = 2) and rubella virus (n = 2) were tested by both ELISA kits to compare their specificity. When «ELISA- IgM-dengue» kits were applied all observed samples were negative, but under the application of the «Anti-Dengue virus ELISA (IgM)» kit (Euroimmun, Germany) 17 samples (32.1%) were false positive or equivocal.

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dengue diagnosis / ELISA-IgM test kits / sensitivity and specificity

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Yu. A Akinshina, V. F Larichev, M. A Saifullin, S. G Mardanly, A. M Butenko. COMPARISON OF THE APPLICATION OF DOMESTIC «ELISA-IGM-DENGUE» KIT, DELIVERED IN THE D.I. IVANOVSKY INSTITUTE OF VIROLOGY (MOSCOW, RUSSIAN FEDERATION) AND «ANTI-DENGUE VIRUS ELISA IGM» KIT (EUROIMMUN, GERMANY) FOR THE SERODIAGNOSIS OF DENGUE FEVER. Epidemiology and Infectious Diseases, 2017, 22(1): 4-8 DOI:10.17816/EID40949

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