IMMUNOLOGICAL PARAMETERSAND BLOOD LYMPHOCYTE ENZYME PROFILE IN BABIES WITH RECURRENT RESPIRATORY INFECTIONS

Abstract

84 babies aged 1 to 3 years suffered from recurrent respiratory infections were studied. Cellular and humoral immunological parameters, phagocytic activity of neutrophils and activities of NAD- and NADP-dependent dehydrogenases in peripheral blood lymphocytes were estimated. Immunophenotypic changes found in blood lymphocytes included decreased IgA levels and a tendency of serum IgM to increase. An elevated level of circulating immune complexes was revealed in the blood serum. Decreased levels active neutrophils in the peripheral blood were detected. In the enzyme status of blood lymphocytes such changes as increased levels of synthetic processes and intense intracellular energy metabolism can be observed.

Keywords

immunity / phagocytosis / enzymes / respiratory infection

References

[1] Альбицкий В.Ю., Баранов А.А. Часто болеющие дети (Клинико-социальные аспекты. Пути оздоровления). -Саратов, 1986.

[2] Калинина Е.В., Чернов Н.Н., Алеид Р. и др. Современные представления об антиоксидантной роли глутатиона и глутатионзависимых ферментов // Вестн. РАМН. - 2010. - № 3. - С. 46-54.

[3] Острые респираторные заболевания у детей: лечение и профилактика // Научно-практическая программа Союза педиатров России. - М., 2002.

[4] Савченко А.А., Сунцова Л.Н. Высокочувствительное определение активности дегидрогеназ в лимфоцитах периферической крови биолюминесцентным методом // Лаб. дело. - 1989. - № 11. - С. 23-25.

[5] Самсыгина Г.А., Казюкова Т.В., Дудина Т.А. и др. Новые технологии в профилактике острых респираторных инфекций у группы детей младшего возраста // Педиатрия. - 2008. - № 5. - С. 102-107.

[6] Современные подходы к лечению и реабилитации часто болеющих детей / Под ред. Л.С. Балевой, Н.А. Коровиной. - М., 2006.

[7] Abbrescia D.I., La Piana G., Lofrumento N.E. Malate- aspartate shuttle and exogenous NADH /cytochrome c electron transport pathway as two independent cytosolic reducing equivalent transfer systems // Arch. Biochem. Biophys. -2012. - Vol. 518, № 2. - P. 157-163.

[8] Bortell R., Moss J., McKenna R.C. et al. Nicotinamide adenine dinucleotide (nad) and its metabolites inhibit T lymphocyte proliferation: role of cell surface nad glycohydrolase and pyrophosphatase activities // J. Immunol. - 2001. - Vol. 167, № 4. - Р 2049-2059.

[9] Boym A. Isolation of lymphocytes from blood and bone marrow // Scand. J. Clin. Lab. Invest. - 1968. - Vol. 21, Suppl. 97. - P. 77-80.

[10] Contreras L., Satrustegui J. Calcium signaling in brain mitochondria: interplay of malate aspartate NADH shuttle and calcium uniporter /mitochondrial dehydrogenase pathways // J. Biol. Chem. - 2009. - Vol. 284, № 11. - Р - 7091-7099.

[11] Garcia-Solis P., Moncada-Alvarez M.C., Martinez-Coria H. et al. Glycerol-3- Phosphate dehydrogenase (E.C.1.1.1.8) is expressed in cultured chicken embryonic adipofibroblasts and upregulated by embryonic chicken serum// Poultry Sci. - 2002. - Vol. 81, № 11. - P. 1709-1713.

[12] Haskova V., Kaslik J., Riha J., Rovensky J. Simple method of circulating immune complex detection in human sera by polyethylene glycol precipitation // J. Immunol. - 1978. -Vol. 154. - P. 399-406.

[13] Kumaraguru U., Rouse R.J., Nair S.K. et al. Involvement of an ATP- dependent peptide chaperone in cross-presentation after DNA immunization // J. Immunol. - 2000. - Vol. 165, N 2. - P. 750-759.

[14] Mancini G., Carbonara A.O., Heremans J.F. Immunochemical quantitation of antigens by single radial diffusion // Im-munochemistry. - 1965. - Vol. 2, N 3. - Р 235-255.

[15] Nogalska A., Pankiewicz A., Goyke E., Swierczynski J. The age-related inverse relationship between ob and lipogenic enzymes genes expression in rat white adipose tissue // Exp. Gerontol. - 2003. - Vol.38, N 4. - P. 415-422.

[16] Norris M.G., Malys N. What is the true enzyme kinetics in the biological system? An investigation of macromolecular crowding effect upon enzyme kinetics of glucose-6-phosphate dehydrogenase // Biochem. Biophys. Res. Commun. - 2011. - Vol. 405, N 3. - P 388-392.

[17] Sorensen M., Skov H., Autrup H. et al. Urban benzene exposure and oxidave DNA damage: influence of genetic polymorphisms in metabolism genes // Sci. Total Environ. - 2003. - Vol. 309, N 1-3. - P. 69-80.

RIGHTS & PERMISSIONS

Eco-vector