Development of a test system for the serodiagnosis of yersiniases by an immunoblot test

Galina Ivanovna Kokorina; Ol'ga Aleksandrovna Burgasova; Galina Petrovna Aniskina; Nikolay Dmitrievich Yushchuk; Galina Yakovlevna Tseneva; G I Kokorina; O A Burgasova; G P Aniskina; N D Yushchuk; G Ya Tseneva

doi:10.17816/EID40554

Epidemiology and Infectious Diseases ›› 2011, Vol. 16 ›› Issue (4) : 18 -23. DOI: 10.17816/EID40554

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Development of a test system for the serodiagnosis of yersiniases by an immunoblot test

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Saint Petersburg Pasteur Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

Moscow State University of Medicine and Dentistry, Ministry of Health and Social Development of Russia

S. P. Botkin Clinical Infectious Diseases Hospital Thirty

Saint Petersburg Pasteur Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

Moscow State University of Medicine and Dentistry, Ministry of Health and Social Development of Russia

S. P. Botkin Clinical Infectious Diseases Hospital Thirty

Moscow State University of Medicine and Dentistry, Ministry of Health and Social Development of Russia

Saint Petersburg Pasteur Research Institute of Epidemiology and Microbiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

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Abstract

The pseudotuberculosis pathogen Y. pseudotuberculosis and the enteric yersiniasis pathogen Y. enterocolitica may cause both acute and chronic diseases. Widely practically used diagnostic methods are effective in the acute period of the diseases whereas diagnosis verification in the protracted and chronic course of yersiniases remains an urgent problem. For solution of this problem, the authors have developed a cost-effective test system for the serodiagnosis of yersiniases by an immunoblot test, by applying the recombinant proteins of recombinant Yersinia outer membrane proteins, such as YopH (51 kD), YopM (44 kD), YopD (35 kD), and YopE (23 kD). As compared to indirect hemagglutination test, agglutination test, and enzyme immunoassay, the immunoblot test has a high sensitivity and a high specificity and may be promising mainly in diagnosing the chronic forms of Yersinia infection.

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Y. pseudotuberculosis / Y. enterocolitica / Y. pseudotuberculosis / Y. enterocolitica / immunoblot / antibodies / serological diagnosis

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Galina Ivanovna Kokorina, Ol'ga Aleksandrovna Burgasova, Galina Petrovna Aniskina, Nikolay Dmitrievich Yushchuk, Galina Yakovlevna Tseneva, G I Kokorina, O A Burgasova, G P Aniskina, N D Yushchuk, G Ya Tseneva. Development of a test system for the serodiagnosis of yersiniases by an immunoblot test. Epidemiology and Infectious Diseases, 2011, 16(4): 18-23 DOI:10.17816/EID40554

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[1]	Бургасова О. А., Куляшова Л. Б., Ценева Г. Я. и др. Сравнительная оценка различных иммунологических реакций в диагностике псевдотуберкулеза // Журн. микробиол. - 1996. - № 2. - С. 48-51.

[2]	Бургасова О. А., Юшук Н. Д., Погорельская Л. В. и др. К дифференциальной диагностике артритов псевдотуберкулезной и хламидийной этиологии // Терапевтический архив. - 2011. - № 10.

[3]	Воротынцева Н. В., Мазанкова Л. Н. Острые кишечные инфекции у детей. - М., 2001.

[4]	Воскресенская Е. А., Козаренко А. А., Ценева Г. Я. Генетические особенности Y. pseudotuberculosis и эпидемиологические черты групповых заболеваний псевдотуберкулезом в организованных коллективах // Эпидемиол. и инфекц. бол. - 2009. - № 3. - С. 31-34.

[5]	Гордеец А. В. Псевдотуберкулез и кишечный иерсиниоз у детей (клиника, диагностика, вопросы патогенеза, лечение): Автореф. дис. ... д-ра мед. наук. - М., 1986. - С. 46.

[6]	Инфекционная заболеваемость в субъектах Российской Федерации за 2008-2009 гг. // Информационный сборник статей и аналитических материалов. - М., 2010. - Ч. 2. - С. 49-52, 76-78.

[7]	Кокорина Г. И., Шендерович О. А., Ценева Г. Я. Применение иммуноблота в диагностике затяжных форм иерсиниозов и изучении вопросов патогенеза (обзор литературы) // Клин. лаб. диагн. - 2006. - № 11. - С. 47-50.

[8]	Королюк А. М. Серологическая диагностика псевдотуберкулеза скарлатиноподобной лихорадки: Автореф. дис. ... канд. мед. наук. - Л., 1972.

[9]	Рябчук Ф. Н., Пирогова З. И. Рецидивы иерсиниозной инфекции как фактор формирования хронической гастроэнтерологической патологии у детей // Гастробюллетень. - 2000. - № 1-2. - С. 81.

[10]	Сомов Г. И., Покровский В. И., Беседнова Н. Н. Псевдотуберкулез. - М., 1990.

[11]	Псевдотуберкулез / Шурыгина И. А., Чеснокова М. В., Климов В. Т. и др. - Новосибирск, 2003.

[12]	Ющук Н. Д., Ценева Г. Я., Кареткина Г. Н., Бродов Л. Е. Иерсиниозы. - М., 2003.

[13]	Harrison's Principles of Internal Medicine / Braunwald E., Fauci A. S., Kasper D. L. et al. - 15-th Ed. - New York, 2001. - P. 993-1001.

[14]	Gaston J. S. H., Lillicrap M. S. Arthritis associated with enteric infection // Best Pract. Res. Clin. Rheumatol. - 2003. - Vol. 17. - P. 219-239.

[15]	Laemli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Nature. - 1970. - Vol. 227. - P. 680-685.

[16]	Leirisalo-Repo M. Yersinia arthritis. Acute clinical picture and long-term prognosis // Contrib. Microbiol. Immunol. - 1987. - Vol. 9. - P. 145-154.

[17]	Paerregaard A., Shand G. H., Gaarslev K. et al. Comparison of crossed immunoelectrophoresis, enzyme-linked immunoassay, and tube agglutination for serodiagnosis of Yersinia enterocolitica serotype O:3 infection // J. Clin. Microbiol. - 1991. - Vol. 29. - P. 302-309.

[18]	Rawlins M. L., Gestner C., Hill H. R. et al. Evaluation of a western blot method for the detection of Yersinia antibodies: Evidence of serological cross-reactivity between Yersinia outer membrane proteins and Borrelia burgdorferi // Clin. Diagn. Lab. Immun. - 2005. - Vol. 12, N 11. - P. 1269-1274.

[19]	Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications // Biotechnology. - 1992. - Vol. 24. - P. 145-149.