Evaluation of the virulence of Vibrio parahaemolyticus strains isolated in Russia and contiguous states

Oksana Andreevna Shalu; E N Golenishcheva; L M Smolikova; G V Gal'tseva; N V Bozhko; E V Monakhova; O A Shalu; Ye N Golenishcheva; L M Smolikova; G V Galtseva; N V Bozhko; Ye V Monakhova

doi:10.17816/EID40496

Epidemiology and Infectious Diseases ›› 2010, Vol. 15 ›› Issue (6) : 45 -48. DOI: 10.17816/EID40496

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Evaluation of the virulence of Vibrio parahaemolyticus strains isolated in Russia and contiguous states

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Abstract

One hundred and ninety-six Vibrio parahaemolyticus strains that are etiological agents causing outbreaks and sporadic cases of food toxicoinfections and isolated from the environments of Russia, Ukraine, and Turkmenia are characterized by virulence-associated phenotypic and genotypic signs. Most clinical strains were found to contain the genes of the major pathogenicity factors - thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH); however, a large number of pathogens lack these genes. In all likelihood, these strains can cause human diseases, by producing other toxic substances. The presence of the trh gene correlated with that and expression of the urease genes. Comparative analysis of the findings has demonstrated that the results of polymerase chain reaction detection of the tdh gene do not always agree with the signs of hemolytic activity on the Wagatsum medium (Kanagawa phenomenon). Therefore, both tests that complement each other are recommended for a fuller evaluation of the potential danger of Vibrio parahaemolyticus strains.

Keywords

Vibrio parahaemolyticus / Vibrio parahaemolyticus / polymerase chain reaction / Kanagawa phenomenon / thermostable direct hemolysin / thermostable direct hemolysin-related hemolysin / urease

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Oksana Andreevna Shalu, E N Golenishcheva, L M Smolikova, G V Gal'tseva, N V Bozhko, E V Monakhova, O A Shalu, Ye N Golenishcheva, L M Smolikova, G V Galtseva, N V Bozhko, Ye V Monakhova. Evaluation of the virulence of Vibrio parahaemolyticus strains isolated in Russia and contiguous states. Epidemiology and Infectious Diseases, 2010, 15(6): 45-48 DOI:10.17816/EID40496

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