PCR detection of the type III secretion system (TTSS) genes and other pathogenicity/ persistence factors in Vibrio cholerae of different serogroups

Abstract

O1, O139, and nonO1/nonO139 Vibrio cholerae strains isolated in Russia, CIS, and other countries underwent polymerase chain reaction screening for their genomic pathogenicity/persistence determinants, including the type III secretion system (TTSS) genes. The TSS genes were first found in V. cholerae O1 serogroup. These genes were shown to be absent in the test toxigenic (ctxAB+) strains of all serogroups and nontoxigenic (ctxAB-) representatives of O139 serogroup, but present in a great deal of ctxAB- strains of O1 and nonO1/nonO139 V. cholerae, including those containing a pre-CTXo/ prophage. Many of the TTSS-positive strains were pathogens of sporadic cases and local outbreaks of non-epidemic cholera. The wide spread of the vcs cluster genes among the clinical and aqueous O1 and nonO1/ nonO139 V. cholera strains suggests the involvement in both their manifestation of virulence and ability to preserve in the environmental objects; however, pathogenicity/persistence factors seem to be interchangeable.

Keywords

Vibrio cholerae / polymerase chain reaction / pathogenicity/persistence factors / type III secretion system

References

[1] Власов В. П., Харланова Н. Г., Монахова Е. В. и др. Ультраструктурные изменения в тонкой кишке, вызванные холерогенным и нехолерогенным штаммами вибрионов // Докл. РАН. - 1997. - Т. 352, № 1. - С. 278-280.

[2] Ерошенко Г. А., Кутырев В. В., Фадеева А. В. и др. Распространение генов системы секреции III типа у холерных вибрионов различных серогрупп // Журн. микробиол. - 2008. - № 4. - С. 23-27.

[3] Монахова Е. В., Писанов Р. В. Токсины холерных вибрионов // Молекул. генетика. - 2005. - № 1. - С. 7-18.

[4] Монахова Е. В., Ломов Ю. М., Михась Н. К., Писанов Р. В. Структура и изменчивость неполного СТХ-элемента холерных вибрионов // Пробл. особо опасных инфекций (Саратов). - 2007. - № 1 (93). - С. 58-61.

[5] Монахова Е. В., Миронова А. В., Алексеева Л. П., Мазрухо А. Б. Изучение вирулентности холерных вибрионов, содержащих preCTXφ: генотипическая и фенотипическая характеристика // Журн. микробиол. - 2008. - № 4. - С. 27- 32.

[6] Онищенко Г. Г., Ломов Ю. М., Москвитина Э. А. и др. Эпидемические проявления холеры, обусловленные ctxAB-tcpA+ холерными вибрионами О1 // Журн. микробиол. - 2007. - № 1. - С. 23-29.

[7] Chatterjee S., Ghoch K., Raychoudhury A. et al. Association of non-O1, non-O139 Vibrio cholerae among hospitalized diarrheal patients at Kolkata: a study on incidence, virulence factors and clonality among clinical strains // J. Clin. Microbiol. - doi: 10.1128/JCM.02026-08.

[8] Chen Y., Johnson J. A., Pusch G. D. et al. The genome of non-Оl Vibrio cholerae NRT3636S demonstrates the presence of pathogenic mechanisms that are distinct from O1 Vibrio cholerae // Infect. and Immun. -2007. - Vol. 75, N 5. - P. 2645-2647.

[9] Dziejman M., Serutto D., Tam V. S. et al. Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system // Proc. Natl. Acad. Sci USA. - 2005. - Vol.102, N 9. - P. 3465-3470.

[10] Faruque S. M., Asadulghani С., Kamruzzaman M. et al. RS1 element of Vibrio cholerae can propagate horizontally as a filamentous phage exploiting the morphogenesis genes of CTX // Infect. and Immun. - 2002. - Vol. 70. - P. 163-170.

[11] Heilpern A. J., Waldor M. K. CTX infection of Vibrio cholerae requires tolQRA gene products // J. Bacteriol. -2000.- Vol. 182. - P. 1739-1747.

[12] Honda T., Nitshibuchi M., Miwatani T., Kaper J. B. Demonstration of a plasmidborn gene encoding a thermostable direct hemolysin in Vibrio cholerae non-O1 strain // Appl. Environ. Microbiol. - 1986. - Vol. 52. - P. 1028-1033.

[13] Jermin W. S., Boyd E. F. Characterization of a novel Vibrio cholerae pathogenicity island (VPI-2) encoding neuraminidase (nanH) among toxigenic Vibrio cholerae isolates // Microbiology. - 2002. - Vol. 148. - P. 3681-3693.

[14] Makino K., Oshima K., Kurokawa K. et al. Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of Vibrio cholerae // Lancet. - 2003. - Vol. 361. - P. 743-749.

[15] Park K. S., Ono T., Rokuda M. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus // Infect. and Immun. - 2004. - Vol. 72. - P. 6659-6665.

[16] Pukazki S., Ma A. T., Sturtvant D. et al. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system // Proc. NatI. Acad. Sci. USA. - 2006. - Vol. 103. - P.1528-1533.

[17] Sears C. L., Kaper J. D. Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion // Microbiol. Rev. - 1996. - Vol. 60. - P. 167-215.

[18] Sheahan K. L., Cordero C. L., Satchell K. J. Identification of a domain within the multiftinctional Vibrio cholerae RTX toxin that covalently cross-links actin // Proc. Nati. Acad. Sci. USA. -2004. - Vol. 101. - P. 9798-9803.

[19] Yahr T. L. A critical new pathway for toxin secretion? // N. Engi. J. Med. -2006. -Vol. 355. - P. 1171-1172.

[20] Yildiz F. H., Schoolnik G. K. Vibrio cholerae O1 El Tor: Identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation // Proc. Natl. Acad. Sci. USA. - 1999. - Vol. 96. - P. 4028-4033.

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