MICRODIALYSIS STUDY OF HIPPOCAMPAL GLUTAMATE AND GABA PROFILE IN RATS AFTER IMMUNIZATION BY GLUTAMATE-BSA CONJUGATE
Alexey E Umryukhin , Natalya Yu Chekmareva , Sergey V Sotnikov , Lyuchia A Vetrile , Irina A Zaharova
Neurology Bulletin ›› 2013, Vol. XLV ›› Issue (1) : 27 -33.
MICRODIALYSIS STUDY OF HIPPOCAMPAL GLUTAMATE AND GABA PROFILE IN RATS AFTER IMMUNIZATION BY GLUTAMATE-BSA CONJUGATE
By means of microdialysis we studied stress induced changes of hippocampal glutamate and GABA profiles in active and passive rats immunized by glutamate-BSA conjugate. Glutamate level after stress exposure at the end of monitored hour period was higher in active immunized rats in comparison to nonimmunized active rats. GABA level during stress exposure significantly increased in active nonimmunized rats. Immunization of activerats by glutamate conjugate was accompanied by an increase of GABA concentration after stress exposure without any changes of GABA during stress exposure. These data demonstrate new conceptions on cerebral neurochemical effects of immunization by glutamate-BSA conjugate.
stress / microdialysis / immunization / glutamate / GABA / dorsal hippocampus / antibodies
| [1] |
1. Данилова Р.А., Ашмарин И.П. Инверсная иммунорегуляция поведения и проблема существования регуляторных аутоантител // Успехи физиол. наук. 1994. Т. 25, № 1. С. 3‒22. |
| [2] |
2. Евсеев В.А. Антитела к нейромедиаторам в механизмах нейроиммунопатологии. М.: Изд-во РАМН, 2007. 148 с. |
| [3] |
3. Коплик Е.В. Метод определения критерия устойчивости крыс к эмоциональному стрессу // Вестн. новых мед. техн.2002. № 1. С. 16-18. |
| [4] |
4. Сергеев П.В., Шимановский Н.Л., Петров В.И. Рецепторы. Волгоград: Семь ветров, 1999. 640 с. |
| [5] |
5. Судаков К.В., Умрюхин П.Е. Системные основы эмоционального стресса. М.: Гэотар-Медиа, 2010. 112 с. |
| [6] |
6. Трекова Н.А., Ветрилэ Л.А., Башарова Л.А. и др. Антитела к дофамину: действие на поведение в «открытом поле», болевую чувствительность, содержание моноаминов в ЦНС и функциональную активность иммуноцитов мышей линии С57Bl/6 // Журн. высш. нервн. деят. 1999. Т. 49, № 5. С.799‒807. |
| [7] |
7. Del Arco A, Segovia G, Fuxe K, Mora F. Changes indialysate concentrations of glutamate and GABA in the brain: an index of volume transmission mediated actions? // J. Neurochem.2003. Vol. 85, N 1. P. 23‒33. |
| [8] |
8. Esposito B., Chandler N., Kandere K. et al. Corticotropin- releasing hormone and brain mast cells regulate blood-brain- barrier permeability by acute stress // J. Pharmacol. Exp. Ther.2002. N. 303. P. 1061‒1066. |
| [9] |
9. Freund T.F., Buzsáki G. Interneurons of the hippocampus // Hippocampus. 1996. Vol. 6, N. 4. P. 347‒470. |
| [10] |
10. Kuang F., Wang B.R., Zhang P. et al. Extravasation of blood-borne immunoglobulin G through blood-brain barrier during adrenaline-induced transient hypertension in the rat // Int. J. Neurosci. 2004. N. 114. P. 575‒591. |
| [11] |
11. Paxinos G., Watson C. The rat brain in stereotaxic coordinates. Oxford: Academic Press, 1998. – 474 p. |
| [12] |
12. Radley J.J., Sawchenko P.E. A common substrate for prefrontal and hippocampal inhibition of the neuroendocrine stress response // J. Neurosci. 2011. Vol. 31, N. 26. P. 9683‒9695. doi: 10.1523/JNEUROSCI.6040-10.2011. |
| [13] |
13. Seguela P., Geffard M., Buijs R.M., LeMoal M. Antibodies against gamma-aminobutyric acid:specificity studies and immunocytochemical results // Proc. Natl. Acad. Sci. 1984. Vol. 81, N. 6. P. 3888‒3892. |
| [14] |
14. Vizi E.S., Kiss J.P. Neurochemistry and pharmacology of the major hippocampal transmitter systems: synaptic and nonsynaptic interactions // Hippocampus. 1998. N. 8. P. 566–607. |
Umryukhin A.E., Chekmareva N.Y., Sotnikov S.V., Vetrile L.A., Zaharova I.A.
/
| 〈 |
|
〉 |
PDF
