THE REACTION OF SOME CD34+- , CD68+- AND CD163+-CELLS IN THE REGENERATION ZONE AFTER APPLYING VARIOUS VACUUM MODES IN CARDIAC SURGERY FOR THE TREATMENT OF STERNOMEDIASTINITIS

G. V. Reva; A. A. Furgal; A. I. Garmash; V. V. Usov; T. Yamamoto; D. D. Kupatadze; V. G. Kozhukhar; E. I. Valkovich; V. K. Verin; I. V. Reva

Morphology ›› 2019, Vol. 156 ›› Issue (6) : 25 -32.

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THE REACTION OF SOME CD34+- , CD68+- AND CD163+-CELLS IN THE REGENERATION ZONE AFTER APPLYING VARIOUS VACUUM MODES IN CARDIAC SURGERY FOR THE TREATMENT OF STERNOMEDIASTINITIS

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Abstract

Objective - a comparative analysis of the mobilization of CD34+-, CD68+- and CD163+-cells in the treatment of sternomediastinitis using various vacuum modes. Materials and methods. Using immunohistochemical and morphometric methods, skin biopsy specimens from the area of post-sternotomy wound healing were studied in 57 patients aged 50 and over who received treatment using continuous (14 patients) or variable vacuum drainage (13 patients), or open method of wound management (30 patients). The localization and number of cells expressing CD34, CD68, CD163, and Ki67 were evaluated in patients of each group in two age categories: 46-60 and 61-75 years old. Results. In all patients age groups, the maximum number of cells expressing CD34, CD68, CD163, and Ki-67 was observed after application of variable vacuum (VAC). The speed of wound cleansing and the active growth of granulation tissue during VAC-therapy with a variable mode of negative pressure occured earlier than in the open management or continuous vacuum drainage. The terms of wound healing were reduced as well as the time spent by patients in the hospital with the absence of subsequent relapses of the inflammatory process. The lowest rates were observed in open management of patients in the age group from 61 to 75 years. Conclusion. The use of a variable vacuum drainage in cardiac surgery is the most effective method for mobilizing CD34+-, CD68+- and CD163+-cells in the treatment of sternomediastinitis.

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CD34+- / sternomediastinitis / CD34+- / CD68+- and CD163+-cells / variable vacuum / wound morphology / regeneration / angiogenesis

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G. V. Reva, A. A. Furgal, A. I. Garmash, V. V. Usov, T. Yamamoto, D. D. Kupatadze, V. G. Kozhukhar, E. I. Valkovich, V. K. Verin, I. V. Reva. THE REACTION OF SOME CD34+- , CD68+- AND CD163+-CELLS IN THE REGENERATION ZONE AFTER APPLYING VARIOUS VACUUM MODES IN CARDIAC SURGERY FOR THE TREATMENT OF STERNOMEDIASTINITIS. Morphology, 2019, 156(6): 25-32 DOI:

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