IMMUNOHISTOCHEMICAL DEMONSTRATION OF APOPTOSIS AND NEUROPLASTICITY IN THE CEREBRAL CORTEX OF ALBINO RAT AFTER OCCLUSION OF THE COMMON CAROTID ARTERIES

Abstract

Objective - to study the activity of the apoptotic proteins (bcl-2, p53, caspase-3) and neuroplasticity (p38, MAP-2) of the sensorimotor cortex (SMC) of the brain of intact albino rats, and at different times after a 20-minute occlusion of common carotid arteries (CCAO). Materials and methods. Light microscopy (staining with hematoxylin and eosin), immunohistochemistry and morphometry were applied. Material for the study: control group (sham-operated animals, n=5), main group (animals 1, 3, 7, 14, 30 days after the CCAO, n=25). Results. It was demonstrated that after CCAO, when an irreversible destruction of part of SMC neurons occurred (layer III - 21,5 %, V - 19,0 %), the processes (MAP-2) and synapses (p38) of the surviving neurons were reorganized. The relative labelling ratio of antibodies to p38 and caspase-3 localized in synaptic terminals was first reduced (on the 1st and 3rd day), and then restored (on the 7th day). In the dynamics of the, The most pronounced changes in caspase-3 during the postischemic period were observed after 7, 14, and 30 days, when its content exceeded the content of p38. Discussion. Post-ischemic compensatory reorganization of the neuron communication system (processes, synapses) is associated with a high content of caspase-3 in axons. No manifestations of apoptosis (activation of caspase-3 in the pericarion) were detected. Conclusions. Caspase-3 must be considered in terms of its pleiotropy, participation in adaptation and recovery processes - neuroplasticity.

Keywords

bcl-2 / acute ischemia / neocortex / caspase-3 / p53 / bcl-2 / p38 / MAP-2

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RIGHTS & PERMISSIONS

Avdeev D.B., Stepanov S.S., Gorbunova A.V., Akulinin V.A., Shoronova A.Y.