THE ASSOCIATION BETWEEN THE VASCULARIZATION OF SKIN REGENERATE AND THE PROLIFERATIVE ACTIVITY OF ITS CELLS

V. G. Shestakova; D. V. Bazhenov; V. V. Banin

Morphology ›› 2017, Vol. 151 ›› Issue (3) : 37 -40.

Articles

research-article

THE ASSOCIATION BETWEEN THE VASCULARIZATION OF SKIN REGENERATE AND THE PROLIFERATIVE ACTIVITY OF ITS CELLS

Author information +

History +

Abstract

Using histological, morphometric and immunohistochemical methods, the correlation between the degree of development of the microcirculatory bed and the level of cell proliferative activity was studied in the healing zone of rat full-layer surgical skin wound. The comparison was made between the results obtained during spontaneous course of the reparative process (control group, n = 20) and under the conditions of angiogenesis stimulation by twofold paravulnary injection of 0.1 ml of Neovasculgene pCMV-VEGF165 plasmid encoding endothelial vascular growth factor (experimental group, n = 20). Morphometric analysis of the material obtained on Days 7, 14 and 21 showed that the number of blood vessels in the newly formed granulation tissue in the experimental group was 1.9 times higher than in the control. Immunohistochemical studies have demonstrated the concentration of proliferative processes in the perivascular zone, especially in animals with stimulated angiogenesis. The use of Neovasculgene resulted in the formation of the denser network of microvessels, the increased cell proliferation, the reduction of overall healing times and the formation of organospecific regenerate.

Keywords

skin / regeneration / stimulated angiogenesis / granulation tissue / proliferation

Cite this article

Download citation ▾

V. G. Shestakova, D. V. Bazhenov, V. V. Banin. THE ASSOCIATION BETWEEN THE VASCULARIZATION OF SKIN REGENERATE AND THE PROLIFERATIVE ACTIVITY OF ITS CELLS. Morphology, 2017, 151(3): 37-40 DOI:

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Алексеева Н. Т., Никитюк Д. Б., Клочкова С. В. Аналитическая морфология репаративной регенерации в коже под действием различных региональных факторов // Журн. анат. гистопатол. 2015. Т. 4, № 1. С. 26-38.

[2]	Банин В. В. Роль перицитов в механизме новообразования сосудов регенерирующей соединительной ткани // Морфология. 2004. Т. 124, вып. 1. С. 45-50.

[3]	Козлов В. И. Капилляроскопия в клинической практике. М.: Практическая медицина, 2015.

[4]	Шестакова В. Г., Банин В. В., Баженов Д. В. Особенности новообразования грануляционной ткани в полнослойной хирургической ране при стимуляции ангиогенеза «Неоваскулгеном» // Журн. анат. гистопатол., 2015. Т. 4. № 3. С. 140.

[5]	Díaz-Flores L. R., Gutiérrez J. F., Madrid H. et al. Pericytes. Morphofunction, interactions and pathologyin a quiescent and activated mesenchymal cell niche // Histol. Histopathol. 2009. Vol. 24. Р. 909-969.

[6]	Hudlicka O., Tyler K. R. Angiogenesis. The Growth of the Vascular System. London: Acad. Press, 1986.

[7]	Silvestre J. S., Tamarat R., Ebrahimian T. G. et al. Vascular endo thelial growth factor-B promotes in vivo angiogenesis // Circ. Res. 2003. Vol. 93. Р. 114-123.