NUCLEOLI IN DEVELOPING HISTAMINERGIC NEURONS OF THE RAT BRAIN

Abstract

Introduction. There are a number of physiological, experimental, and pathological conditions that can induce changes in the size, morphology, location, and number of nucleoli in accordance with functional and metabolic activity. One of these conditions is the postnatal maturation of cells, including neurons. Objective - to assess the characteristics of structural and functional formation of histaminergic neurons nucleoli in rat brain during postnatal ontogeny. Material and methods. The work was performed on the offspring of outbred white rats (12 rats) on the 5th, 20th and 45th days of postnatal ontogenesis. Electron microscopic, morphometric and statistical methods were used to study the number and quantity of nucleoli in the nuclei of histaminergic neurons located in the E2 nucleus of posterior hypothalamic region of rat brain. Results. From the 5th to the 45th day of the rat postnatal development, there was a decrease in the number and increase in the size of the nucleoli in the nuclei of histaminergic neurons located in the posterior hypothalamic region, as well as their movement from the karyolemma to the center of the nucleus. As animals growed up, relatively compact nucleoli in the brain histaminergic neurons gradually transformed into reticular nucleoli, while the quantity of fibrillar and granular components increased, at the same time the quantity of fibrillar centers increased and the size of fibrillar centers decreased. In addition, we detected a decrease in the cloud of migrating ribosome subunits between the nucleolus and karyolemma and in the amount of heterochromatin associated with the nucleolus. Conclusions. From the 5th to the 45th day of the postnatal development, the number, size, structure and topography of the nucleoli of the rat brain histaminergic neurons located in the E2 hypothalamic nucleus in postnatal ontogenesis changed significantly.

Keywords

hypothalamic histaminergic neurons / nucleoli / rat brain / postnatal ontogenesis

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RIGHTS & PERMISSIONS

Zimatkin S.M., Zaerko A.V., Fedina K.M.