CHANGES IN HEMODYNAMICS, ENERGY METABOLISM AND PLASTIC PROCESSES IN THE LIVER IN ACUTE SMALL BOWEL OBSTRUCTION CAUSED BY OBTURATION

Abstract

Objective - to reveal the patterns of morpho-functional changes in the liver in the dynamics of the development of acute small bowel obstruction (ASBO) caused by obturation. Material and methods. The study was performed on 18 adult mongrel dogs of both sexes weighing 17-20 kg, in which low small bowel obstruction was modeled. All scientific experiments were conducted in accordance with the guidance documents, guidelines for the care and use of laboratory animals of the National Institute of Health (National Institute of Health - NIH, Bethesda, USA) and the «Rules for working with experimental animals», approved by the Ethics Committee for Federal State Institution «Main Military Clinical Hospital named after Academician N. N. Burdenko» of the Ministry of Defense of the Russian Federation and the local ethics committee of the I. M. Sechenov First Moscow State Medical University. On histological sections stained with hematoxylin and eosin, the change in the area of the vascular bed of the liver was evaluated, the glycogen content in the liver was determined by a quantitative evaluation of the products of the Schick reaction and the level of total and cytoplasmic nucleoprotein according to Einarsson. Results. From the 3rd day after the formation of an obstruction, the central venous area decreased by 3.15 times compared with the norm, and the area of interlobular veins and arteries increased by 1.49 times and 1.55 times, respectively. The level of glycogen and nucleoproteins remained reduced throughout the experiment. Conclusions. In the formation of obturational ASBO, despite the absence of its clinical manifestations, a change in the organization of hemodynamics was noted since the 3rd day of the operation. It indicated the formation of the anastomotic hemodynamic pathway, bypassing the liver, which is the basis for reducing the detoxification function of the liver. It was also accompanied by energy and protein-synthetical liver dysfunction, which is the morpho-functional basis for the development of multiple organ dysfunction syndrome (MODS).

Keywords

acute small bowel obstruction / energy metabolism / plastic processes / hemocirculatory bed of liver

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RIGHTS & PERMISSIONS

Milyukov V.Y., Sharifova H.M.