SUBPOPULATIONS OF CALBINDIN-, CALRETININ- AND PARVALBUMINIMMUNOREACTIVE INTERNEURONS OF THE DORSAL HORN OF THE SPINAL CORD OF FEMALE C57BL/6 MICE

Abstract

Objective - to make a comparative analysis of interneurons subpopulations that contain calbindin (CAB), calretinin (CAR) and parvalbumin (PAV) in the dorsal horn of the TIII-TV segments of the spinal cord (SC). Materials and methods. Immunoreactive (IR) interneurons in 16-week-old female C57BL/6 mice were studied using immunohistochemical methods. Results. All subpopulations of IR interneurons were located in all laminae of the dorsal horn of the SC; however, interneurons IR to PAV were not detected in plate I. The relative content of CAB interneurons was greatest in laminae I (27 %) and II (29 %), CAR interneurons - in lamina II (21,5 %), PAV interneurons - in laminae IV (5,7 %) and V (6,2 %). In addition, a subpopulation of PAV interneurons in plates II, III and in the region of the dorsal horn medial border (MB) was represented by a small group of cells, in contrast with other calcium-binding proteins. The same was true for CAR interneurons in plates III, IV, V and MB. The quantitative distribution of the subpopulation of calbindin-containing interneurons prevailed in all laminae of the dorsal horn of SC. The differences in cell sizes in the mentioned subpopulations of IR interneurons were statistically significant, with CAB and CAR interneurons being larger and PAV interneurons - smaller. Conclusions. In the dorsal horn of the SC, various subpopulations of interneurons immunoreactive to calbindin, calretinin, and parvalbumin were identified, and the subpopulations were specific in each lamina.

Keywords

interneuron / dorsal horn / spinal cord / immunohistochemistry

References

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RIGHTS & PERMISSIONS

Porseva V.V., Emanuilov A.I., Masliukov P.M.