Immunohistochemical study of the nervous structures of the mediastinal organs

Abstract

BACKGROUND: No studies have sufficiently evaluated the innervation of the mediastinal organs in the P1 period when the blood circulation switches from placental to pulmonary and the regulation of respiration and functioning of the cardiovascular system begin.

AIM: This study aimed to conduct a comparative immunohistochemical analysis of the innervation of the mediastinal organs of newborn rats.

MATERIALS AND METHODS: Immunohistochemical methods were employed for identifying neuronal markers: PGP 9.5 protein, tyrosine hydroxylase, and synaptophysin. Primary antibodies were applied to mediastinal sections after they had been deparaffinized.

RESULTS: Analysis of frontal sections of the cardiopulmonary complex of rats at P1 showed that the bronchi, esophagus, great vessels (aorta and pulmonary artery trunks), veins (pulmonary and caval), adipose tissue, lymph nodes, lung fragments, and heart occupied the upper and middle sections of the mediastinum. Closely adjacent parasympathetic and sympathetic ganglia, microganglia, nerve trunks, and plexuses of nerve fibers were identified using neuronal markers in the interstitium between organs. In the upper and middle mediastinum of newborn rats, synaptic structures were identified in the ganglia and paraganglia, mixed lobules of adipose tissue, esophageal and bronchial wall, around small arteries and arterioles for the first time using immunohistochemical methods.

CONCLUSION: This comparative study revealed that in the early period of postnatal development of rats, the walls of the esophagus, trachea, and main bronchi are most innervated in the mediastinum; in the myocardium and lungs, these nervous apparatuses are typically rare or absent. A high concentration of synaptic structures in the conducting myocardium has been described.

Keywords

mediastinal organs / newborn rat / innervation / PGP 9.5 protein / tyrosine hydroxylase / synaptophysin / immunohistochemistry

References

[1] Shvalev VN, Sosunov AA, Guski G. Morphological basis of heart innervation. Moscow: Nauka; 1992. (In Russ).

[2] Швалев В.Н., Сосунов А. А., Гуски Г. Морфологические основы иннервации сердца. Москва: Наука, 1992.

[3] Chumasov EI. On the issue of blood supply and innervation of the aortic-pulmonary or pericardial chromaffin paraganglia of the cat. Arhiv anatomii, gistologii i jembriologii. 1971;61(7):92–99. (In Russ). EDN: YOXGQL

[4] Чумасов Е.И. К вопросу о кровоснабжении и иннервации аортально-пульмональных или околосердечных хромаффинных параганглиев кошки // Архив анатомии, гистологии и эмбриологии. 1971. Т. 61, № 7. С. 92–99. EDN: YOXGQL

[5] Nozdrachev AD, Chumasov EI. Peripheral nervous system. Saint Petersburg: Nauka; 1999. 281 p. (In Russ). EDN: VZLWTL

[6] Ноздрачев А.Д., Чумасов Е. И. Периферическая нервная система. Санкт-Петербург: Наука, 1999. 281 с. EDN: VZLWTL

[7] Bőck P. The paraganglia. Berlin etc.: Springer; 1982. doi: 10.1007/978-3-642-68208-7

[8] Bőck P. The Paraganglia. Berlin etc.: Springer, 1982. doi: 10.1007/978-3-642-68208-7

[9] Nozdrachev AD. Physiology of the autonomic nervous system. Minsk: Medicine; 1983. (In Russ).

[10] Ноздрачев А. Д. Физиология вегетативной нервной системы. Минск: Медицина, 1983.

[11] Dudanov IP, Pigarevsky PV, Korgevsky DE, et al. Atherosclerosis, diabetes mellitus and autonomic innervation of the cardiovascular system. Medical Academic Journal. 2012;12(2):19–27. EDN: QBXHSJ

[12] Дуданов И.П., Пигаревский П. В., Коржевский Д. Э., и др. Атеросклероз, сахарный диабет и автономная иннервация органов сердечно-сосудистой системы // Медицинский академический журнал. 2012. Т. 12, № 2. С. 19–27. EDN: QBXHSJ

[13] Shvalev VN, Reutov VP, Rogoza AN, et al. Development of modern ideas about the neurogenic nature of cardiac diseases. Pacific Medical Journal. 2014;(1):10–14. EDN: SNMJAR

[14] Швалев В.Н., Реутов В. П., Рогоза А. Н., и др. Развитие современных представлений о нейрогенной природе кардиологических заболеваний // Тихоокеанский медицинский журнал. 2014. № 1. С. 10–14. EDN: SNMJAR

[15] Korzhevskii DE, Kirik OV, Petrova ES, et al. Theoretical foundations and practical application of immunohistochemistry methods (manual). 2nd edition. Saint Petersburg: SpetsLit; 2014. 118 p. (In Russ). EDN: SINOMT

[16] Коржевский Д.Э., Кирик О. В., Петрова Е. С., и др. Теоретические основы и практическое применение методов иммуногистохимии (руководство). 2-е издание. 118 с. Санкт Петербург: СпецЛит, 2014. EDN: SINOMT

[17] Chumasov EI, Kolos EA, Petrova ES, Korzhevskii DE. Immunohistochemistry of the peripheral nervous system. Saint Petersburg: SpetsLit; 2020. 111 p. (In Russ). EDN: FUSCZP

[18] Чумасов Е.И., Колос Е. А., Петрова Е. С., Коржевский Д. Э. Иммуногистохимия периферической нервной системы. Санкт Петербург: СпецЛит, 2020. 111 с. EDN: FUSCZP

[19] Nagornev VA, Chumasov EL, Korzhevskii DE, et al. Modern approaches to the study of the nervous apparatus of arteries in atherosclerosis and diabetes. Medical Academic Journal. 2010;10(3):19–27. EDN: TIQRGX

[20] Нагорнев В.А., Чумасов Е. И., Коржевский Д. Э., и др. Современные подходы к изучению нервных аппаратов артерий при атеросклерозе и сахарном диабете // Медицинский академический журнал. 2010. Т. 10, № 3. С. 19–27. EDN: TIQRGX

[21] Chumasov E, Alekseenko A, Petrova E, Korzhevsky D. Study of the nervous apparatus of the heart of rats of different ages using immunohistochemical markers. International Bulletin of Veterinary Medicine. 2017;(1):41–46. EDN: YIPFWZ

[22] Чумасов Е.И., Алексеенко А. Л., Петрова Е. С., Коржевский Д. Э. Исследование нервных аппаратов сердца крыс различного возраста с помощью иммуногистохимических маркеров // Международный вестник ветеринарии. 2017. № 1. С. 41–46. EDN: YIPFWZ

[23] Grigoriev IP, Korzhevskii DE. Current technologies for fixation of biological material for immunohistochemical analysis (review). Sovremennye tehnologii v medicine [Modern Technologies in Medicine]. 2018;10(2):156–165. EDN: XRVMWL doi: 10.17691/stm2018.10.2.19

[24] Григорьев И.П., Коржевский Д. Э. Современные технологии фиксации биологического материала, применяемые при проведении иммуногистохимических исследований (обзор) // Современные технологии в медицине. 2018. Т. 10, № 2. С. 156–165. EDN: XRVMWL doi: 10.17691/stm2018.10.2.19

[25] Suster S, Moran CA, Dominguez-Malagon H, Quevedo-Blanco P. Germ cell tumors of the mediastinum and testis: a comparative immunohistochemical study of 120 cases. Hum Pathol. 1998;29(7):737–742. doi: 10.1016/s0046-8177(98)90284-2

[26] Suster S., Moran C. A., Dominguez-Malagon H., Quevedo-Blanco P. Germ cell tumors of the mediastinum and testis: a comparative immunohistochemical study of 120 cases // Hum Pathol. 1998. Vol. 29, N 7. P. 737–742. doi: 10.1016/s0046-8177(98)90284-2

[27] Nasit JG, Patel M, Parikh B, et al. Anterior mediastinal masses: a study of 50 cases by fine needle aspiration cytology and core needle biopsy as a diagnostic procedure. South Asian J Cancer. 2013;2(1):7–13. doi: 10.4103/2278-330X.105872

[28] Nasit J.G., Patel M., Parikh B., et al. Anterior mediastinal masses: a study of 50 cases by fine needle aspiration cytology and core needle biopsy as a diagnostic procedure // South Asian J Cancer. 2013. Vol. 2, N 1. P. 7–13. doi: 10.4103/2278-330X.105872

[29] Mondal P, Nag D, Samaddar A, et al. Clinicopathological spectrum of anterior mediastinal lesions with special reference to the role of cytology in diagnosis: a cross-sectional study. Natl J Physiol Pharm Pharmacol. 2022;12(6):880–885. doi: 10.5455/njppp.2022.12.11414202102122021

[30] Mondal P., Nag D., Samaddar A., et al. Clinicopathological spectrum of anterior mediastinal lesions with special reference to the role of cytology in diagnosis: a cross-sectional study // Natl J Physiol Pharm Pharmacol. 2022. Vol. 12, N 6. P. 880–885. doi: 10.5455/njppp.2022.12.11414202102122021

[31] Vemuluri M, Philipose TR. Histopathological study and immunohistochemistry of mediastinal masses of anterior compartments. Annals of Pathology and Laboratory Medicine. 2023;10(1): A1–A12. doi: 10.21276/APALM.3210

[32] Vemuluri M., Philipose T. R. Histopathological study and immunohistochemistry of mediastinal masses of anterior compartmen // Annals of Pathology and Laboratory Medicine. 2023. Vol. 10, N 1. P. A1–A12. doi: 10.21276/APALM.3210

[33] Sundaram S, Vidhyalakshmi S. Histomorphological spectrum of mediastinal masses with special emphasis on rarelesions. Journal of Clinical and Diagnostic Research. 2020;14(8):1–5. doi: 10.7860/JCDR/2020/44483.13884

[34] Sundaram S., Vidhyalakshmi S. Histomorphological spectrumof mediastinal masses with special emphasis on rarelesions // Journal of Clinical and Diagnostic Research. 2020. Vol. 14, N 8. P. 1–5. doi: 10.7860/JCDR/2020/44483.13884

[35] Akutsu Y, Kaneko K, Kodama Y, et al. Usefulness of severe cardiac sympathetic dysfunction to predict the occurrence of rapid atrial fibrillation in patients with Wolff-Parkinson-White syndrome. Am J Cardiol. 2013;112(5):688–693. doi: 10.1016/j.amjcard.2013.04.048

[36] Akutsu Y., Kaneko K., Kodama Y., et al. Usefulness of severe cardiac sympathetic dysfunction to predict the occurrence of rapid atrial fibrillation in patients with Wolff-Parkinson-White syndrome // Am J Cardiol. 2013. Vol. 112, N 5. P. 688–693. doi: 10.1016/j.amjcard.2013.04.048

[37] Bulygin IA. Afferent link of interoceptive reflexes. Minsk: Medicine; 1971. (In Russ).

[38] Булыгин И. А. Афферентное звено интероцептивных рефлексов. Минск: Медицина, 1971.

[39] Samoilov VO. Some problems of carotid chemoreception. In: Gershuni GV, editor. Sensory systems. Leningrad: Nauka; 1977. P. 76–86. (In Russ).

[40] Самойлов В. О. Некоторые проблемы каротидной хеморецепции. В кн.: Сенсорные системы / под ред. Г. В. Гершуни. Ленинград: Наука, 1977. С. 76–86.

[41] Ilyinsky OB, Kislyakov VA, Levashov MM. Physiology of sensory systems. Gershuni GV, editor. Part 2. Leningrad: Science; 1972. (In Russ).

[42] Ильинский О.Б., Кисляков В. А., Левашов М. М., и др. Физиология сенсорных систем. Ч. 2 / отв. ред. Г.В. Гершуни. Ленинград: Наука, 1972.

[43] Floras JS. Arterial baroreceptor and cardiopulmonary reflex control of sympathetic outflow in human heart failure. Ann N Y Acad Sci. 2001;940:500–513. doi: 10.1111/j.1749-6632.2001.tb03701.x

[44] Floras J. S. Arterial baroreceptor and cardiopulmonary reflex control of sympathetic outflow in human heart failure // Ann N Y Acad Sci. 2001. Vol. 940. P. 500–513. doi: 10.1111/j.1749-6632.2001.tb03701.x

[45] Krieger EM, Da Silva GJ, Negrão CE. Effects of exercise training on baroreflex control of the cardiovascular system. Ann NY Acad Sci. 2001;940:338–347. doi: 10.1111/j.1749-6632.2001.tb03689.x

[46] Krieger E.M., Da Silva G. J., Negrão C. E. Effects of exercise training on baroreflex control of the cardiovascular system // Ann NY Acad Sci. 2001. Vol. 940. P. 338–347. doi: 10.1111/j.1749-6632.2001.tb03689.x

[47] Gaivoronsky IV, Zheleznov LM, Yakhina IM. Topographical and anatomical features of the esophagus of human fetuses 16–22 weeks of development. In: Questions of morphology of the XXI century. Issue 8. Saint Petersburg: DEAN Publishing House; 2024. P. 143–148. (In Russ).

[48] Гайворонский И.В., Железнов Л. М. Яхина И. М. Топографо-анатомические особенности пищевода плодов человека 16–22 недель развития. В кн.: Вопросы морфологии ХХI века. Выпуск 8. Санкт-Петербург: Издательство ДЕАН, 2024. С. 143–148.

[49] Gershon MD. The enteric nervous system: a second brain. Hosp Pract (1995). 1999;34(7):31–32,35–38,41–42 passim. doi: 10.3810/hp.1999.07.153

[50] Gershon M. D. The enteric nervous system: a second brain // Hosp Pract (1995). 1999. Vol. 4, N 7. P. 31–32, 35–38, 41–42 passim. doi: 10.3810/hp.1999.07.153

[51] Furness JB, Stebbing MJ. The first brain: species comparisons and evolutionary implications for the enteric and central nervous systems. Neurogastroenterol Motil. 2018;30(2):10.1111/nmo.13234. doi: 10.1111/nmo.13234

[52] Furness J.B., Stebbing M. J. The first brain: species comparisons and evolutionary implications for the enteric and central nervous systems // Neurogastroenterol Motil. 2018. Vol. 30, N 2. P. 10.1111/nmo.13234. doi: 10.1111/nmo.13234

[53] Chumasov EI, Petrova ES, Korzhevskii DE. Morphological peculiarities features of the innervation of rat epicardial adipose tissue in early postnatal ontogenesis. Journal of Evolutionary Biochemistry and Physiology. 2022;58(6):2070–2079. doi: 10.1134/S0022093022060333

[54] Chumasov E.I., Petrova E. S., Korzhevskii D. E. Morphological peculiarities features of the innervation of rat epicardial adipose tissue in early postnatal ontogenesis // Journal of Evolutionary Biochemistry and Physiology. 2022. Vol. 58, N 6. P. 2070–2079. doi: 10.1134/S0022093022060333

Funding

Федеральное государственное бюджетное научное учреждение «Институт экспериментальной медицины»Institute of Experimental Medicine(122020300199-5)

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